H400 Cell Line

Established from a squamous cell carcinoma (SCC) of the alveolar process (20mm – 40mm) of a female patient aged 55. STNMP stage II, moderately differentiated, node negative tumour.

Contributors

Inventor Institute
Stephen Prime University of Bristol
Cat. #: 153424
Unit size: 1×10^6 cells / vial
Research Fields: Cancer
Organism: Human
Tissue: The alveolar process of the maxilla
Gender: Female
Model: Tumour cell line
Growth properties: Adherent
Primary citation: Prime et.al. 1990. J Pathol. 160(3):259-69. PMID: 1692339
Alternate name: h400; H-400
Product description: Established from a squamous cell carcinoma (SCC) of the alveolar process (20mm – 40mm) of a female patient aged 55. STNMP stage II, moderately differentiated, node negative tumour. This cell line is highly responsive to TGF-beta. These cells are non-tumourigenic on subcutaneous injection into athymic nude mice, but tumourigenic on injection into the floor of the mouth
Gender: Female
CRISPR: No
Conditional: No
Additional notes: Haplotype information: A*11,A*29; B*07,B*15; Cw*03,Cw*15
Cellosaurus ID: CVCL_2464
Model description: Mutant p53, codon 283 exon 8, C to G wild type K-, N- and Ha-ras. Non-tumourigenic on subcutaneous injection into athymic nude mice, but tumourigenic on injection into the floor of the mouth.
Disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
Subculture routine: Split sub-confluent cultures (70-80%) 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37C. Suggested seeding density 5 x 1000 cells/cm2. Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability
Temperature: 37° C
Atmosphere: 5% CO2
Mycoplasma free: Yes
References:

Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286

Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893

Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215

Yeudall et.al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-7. PMID: 8180579

Prime et.al. 1990. J Pathol. 160(3):259-69. PMID: 1692339

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