Cat. #151556
UNG Mouse
Cat. #: 151556
Sub-type: Mouse
Availability: 8-10 weeks
Disease: Cancer
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Tomas Lindahl
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: UNG Mouse
- Cancer: Blood cancer
- Cancers detailed: B cell lymphoma
- Research fields: Cancer;Genetics;Immunology
- Tool sub type: Mouse
- Disease: Cancer
- Conditional: No
- Description: In vivo study of UNG knockout and DNA mismatch mutation during DNA synthesis. Mice develop B cell lymphoma and are abnormally defective in their immune response. There are also mouse embryonic fibroblasts from this line available.
- Genetic background: A ung targeting vector, replacing exon 4 with a resistance cassette, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into C57BL6 blastocysts. Chimeric mice were mated with C57BL6 mice to generate heterozygotes. Heterozygous mice were crossed to generate homozygous Ung-/- mice.
- Zygosity: Homozygous
- Production details: A ung targeting vector, replacing exon 4 with a resistance cassette, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into C57BL6 blastocysts. Chimeric mice were mated with C57BL6 mice to generate heterozygotes. Heterozygous mice were crossed to generate homozygous Ung-/- mice.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Uracil-DNA Glycosylase (UNG)
References
- Nilsen et al. 2003. Oncogene. 22(35):5381-6. PMID: 12934097.
- Gene-targeted mice lacking the Ung uracil-DNA glycosylase develop B-cell lymphomas.
- Nilsen et al. 2000. Mol Cell. 5(6):1059-65. PMID: 10912000.
- Uracil-DNA glycosylase (UNG)-deficient mice reveal a primary role of the enzyme during DNA replication.
