Cat. #151448
CMT 167 Cell Line
Cat. #: 151448
Sub-type: Primary
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Mouse
Tissue: Lung
Disease: Cancer
Model: Tumour line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Peter Riddle
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: CMT 167 Cell Line
- Cancer: Lung cancer
- Cancers detailed: Lung carcinoma
- Research fields: Cancer;Drug development
- Tool sub type: Primary
- Organism: Mouse
- Gender: Female
- Tissue: Lung
- Disease: Cancer
- Growth properties: Tumourigenesis
- Model: Tumour line
- Conditional: Yes
- Description: Highly metastatic murine alveogenic lung carcinoma cell line, derived from cell line CMT64, used to study the origins and causes of metastasis. <Background and Research Application: CMT167 was created in 1984 at Cancer research UK London Research Institute: Lincolns Inn Fields as a cell line with increased metastatic capabilities (selected from pooled lung metastases) from the CMT64 cell line. CMT 167 was isolated by subcloning and in vivo screening for high metastatic potential. CMT167 is a cell line of higher metastatic potential in comparison to CMT64CMT 167 is an epithelial cell line, with closely packed sheets at confluence. It has an adherent culture type.
- Production details: Mouse; subclone of CMT 64 alveogenic lung carcinoma (isolated from primary tumour in C57BL/lcrf mouse), CMT 167 isolated by subcloning and in vivo screening for high metastatic potential. Production Details: Adult female mice (4-6 months) C57B/T were subcutaneously injected in the lower right flank with pooled tumour fragments from different non-necrotic areas of tumour with a Bashford needle - the cells from these tumours are on CMT64 origin. These cells were then cultured from lung metastases and the epithelial outgrowths were established as CMT167.
- Cellosaurus id: CVCL_2405
Handling
- Format: Frozen
- Growth medium: DMEM with 2mM Glutamine and 10% FCS
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 10^4 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density 1.2 x 10^5 cells/cm²
Related Tools
- Related tools: CMT 64/61 Cell Line
References
- Tippimanchai et al. 2018. Oncoimmunology. 7(6):e1438105. PMID: 29872579.
- Seshadri et al. 2018. Front Pharmacol. 9:759. PMID: 30061830.
- Pelaz et al. 2017. ACS Nano. 11(3):2313-2381. PMID: 28290206.
- Ismail et al. 2000. Cancer Res. 60(5):1173-6. PMID: 10728668.
- Layton et al. 1984. Br J Cancer. 49(4):415-21. PMID: 6324836.
- Heterogeneity in a spontaneous mouse lung carcinoma: selection and characterisation of stable metastatic variants.
- Franks et al. 1976. Cancer Res. 36(3):1049-55. PM...
