Cat. #161591
IPTG-inducible pGEX-6P1 expression vector for GST and human CB1 receptor (AA 414 to 442) fusion protein
Cat. #: 161591
Sub-type: IPTG-inducible pGEX-6P1 expression vector
Bacterial Resistance: Ampicillin
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Gontzal GarcĂa del CaĂąo
Institute: University of the Basque Country
Primary Citation: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
Tool Details
*FOR RESEARCH USE ONLY
- Tool name: IPTG-inducible pGEX-6P1 expression vector for GST and human CB1 receptor (AA 414 to 442) fusion protein
- Tool sub type: IPTG-inducible pGEX-6P1 expression vector
- Sequence: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
- Insert: GST-CB1414â442
- Tag: glutathione S-transferase (GST)
- Total vector size: 5048 bp
- Backbone size without insert: 4985 bp
- Insert size: 93 bp
- Insert species: Human
- Promoter: Isopropyl ?-D-thiogalactoside (IPTG)-inducible tac promoter. The vector is designed with an internal lacIq gene whose protein product binds to the operator region of the tac promoter. Thus, expression is repressed unless IPTG is added to the culture, thus maintaining tight control over the expression of the insert.
- Bacterial resistance: Ampicillin
- Description: Purified plasmid DNA containing the IPTG inducible coding sequence for the polypeptide spanning amino acids 414 to 442 of the cytosolic tail of the human CB1 receptor fused to the C-terminus of the glutathione S-transferase tag (GST-CB1414â442)
- Application: GST fusion protein production
- Cloning information: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
- Additional notes: Contact the inventor for further information.
Handling
- Storage conditions: Refrigerated at 4 oC (or frozen at -20 oC for long periods)
- Storage buffer: Double distilled water
- Shipping conditions: At room temperature in water solution
Application Details
- Application: GST fusion protein production
- Application notes: GST fusion protein production
References
- GĂłmez-Caballero et al. (2021). Mikrochim Acta, 188:368. doi:10.1007/s00604-021-05029-z
