pKH2 Beta-Synuclein P123H Vector

pKH2 (“P123H”) is a derivative of pET15b with the open reading frame encoding the P123H mutant human beta-synuclein (β-synuclein) cloned in via the vector NdeI and BamHI restriction sites. It was constructed via site-specific mutagenesis of pJEK12 (pET15b-wt β-synuclein), replacing the C at position 368 in the ORF nucleotide sequence with A, thus altering the 123rd codon from CCC encoding proline (P) to CAC encoding histidine (H). In this construct P123H β-synuclein is expressed as a fusion protein with an N-terminal six His tag.

Contributors

Inventor Institute
Dr Fiona Benson Lancaster University
SKU: 152053
Product description: pKH2 ("P123H") is a derivative of pET15b with the open reading frame encoding the P123H mutant human beta-synuclein (β-synuclein) cloned in via the vector NdeI and BamHI restriction sites. It was constructed via site-specific mutagenesis of pJEK12 (pET15b-wt β-synuclein), replacing the C at position 368 in the ORF nucleotide sequence with A, thus altering the 123rd codon from CCC encoding proline (P) to CAC encoding histidine (H). In this construct P123H β-synuclein is expressed as a fusion protein with an N-terminal six His tag.
Additional notes: Beta synuclein is an abundant pre-synaptic phosphoprotein that is found in the brain and is homolgous to alpha-synuclein. Beta-synuclein is distinct from alha-synuclein in that it lacks the majority of the hydrophobic non-amyloid-beta component of the Alzeheimer's disease amyloid region. Due to this beta-synuclein is less likely to form insoluble aggregates when compared to alpha-synuclein. It is thought that beta-synuclein may have a protective role against alpha-synucleinopathies. Overexpression of beta-synuclein mutants (P123H and V70M) in neuroblastoma cells results in enhanced lysosomal pathology suggesting a causative role for these missense mutations in neurodegeneration stimulation
Cat. #: 152053
Research Fields: Neurobiology
Target: Beta-synuclein
Application notes: pKH2 ("P123H") is a derivative of pET15b with the open reading frame encoding the P123H mutant human beta-synuclein (β-synuclein) cloned in via the vector NdeI and BamHI restriction sites. It was constructed via site-specific mutagenesis of pJEK12 (pET15b-wt β-synuclein), replacing the C at position 368 in the ORF nucleotide sequence with A, thus altering the 123rd codon from CCC encoding proline (P) to CAC encoding histidine (H). In this construct P123H β-synuclein is expressed as a fusion protein with an N-terminal six His tag.

Images

View Gallery

Related Tools for Vectors

Cat. # Tool Name
151830 MultiDsk Vector Key Info View Tool
152051 pJEK6 Alpha-Synuclein E46K Vector Key Info View Tool
152594 Norrin Mutant [R107E/R109E/R115L] Vector Key Info View Tool
152742 Chicken IgY precipitating protein Vector Key Info View Tool
153287 pCMV-iNOS Vector Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.