Instituto de Medicina Molecular Jo?Âo Lobo Antunes
SKU:
160727
Product description:
Deletion of mouse Xist lncRNA to dissect specific RNA modules in the process of X-chromosome inactivation.
Conditional:
No
Conditional description:
Inducible through addition of doxycycline (1.5 ÄÂÄ?g/ml) to the medium
Production details:
CRISPR/Cas9 genome editing of the Xist-TetOP XY ESC line
Additional notes:
Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Ca…
Parental cell line:
Xist-TetOP XY ESCs (Wutz et al., 2002 PMID: 11780141)
Cat. #:
160727
Tool sub type:
Continuous
Unit size:
1×10^6 cells / vial
Research Fields:
Genetics; Stem cell biology
Organism:
Mouse
Model:
Stem Cells
Growth properties:
No disruption of Polycomb recruitment to the inactive X-chromosome.
Format:
Frozen
Shipping conditions:
Dry ice
Growth medium:
ESCs grow in feeder-free classic ESC medium DMEM media containing 15% fetal bovine serum (FBS), 103 U/ml leukemia inhibitor factor (LIF), 10-4 mM 2-mercaptoethanol, 50 U/ml penicillin, and 50 ?g/ml of streptomycin (Gibco) at 37?°C in 5-8% CO2. Medium is changed daily Inducible expression of Xist driven by a TetO promoter is achieved by adding DOX (1.5 ?g/ml).
Recommended controls:
Xist-TetOP XY ESCs (Wutz et al., 2002 PMID: 11780141)
References:
Bousard et al. 2019. EMBO Rep. 20(10):e48019. PMID: 31456285.
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