SK-MES-1 is a human lung cancer cell line that displays epithelial morphology and grows as monolayers in tissue culture.
Products or characteristics of interest:
Karyotype: The stemline chromosome number is hypotriploid, with the 2S component occurring at 3.2%. Seventeen to 20 marker chromosomes were common to most S metaphases. Normal X, 13, and 19 chromosomes were absent, and chromosomes 2, 3, 14, 17 and 20 were generally monosomic. The Y chromosome was not detected using QM staining. Isoenzymes: AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1-2 PGM1, 1-2 PGM3, 1
Cat. #:
161935
Cancer types:
Lung cancer
Tissue:
Lung
Morphology:
Epithelial
Growth properties:
Adherent
Primary citation:
Fogh et al. 1977. Journal of the National Cancer Institute. 58.2.209. PMID: 833871.
Shipping conditions:
Dry Ice
Growth medium:
1:1 mixture of ATCC-formulated Eagle’s Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium then supplemented with fetal bovine serum to a final concentration of 10%.
Temperature:
37° C
Atmosphere:
5% CO2 in air
Storage medium:
Complete growth medium supplemented with 5% DMSO
Biosafety level:
1
References:
Blobel et al. 1984. Virchows Arch B Cell Path Incl Mol Pathol. 45(4):407-429. PMID: 6203212.
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