OV-3133(R) cell line

Relapse-derived HGSOC ascites cell line from the matched 3133 series for studying disease after treatment

Contributors

Inventor Institute
Anne-Marie Mes-Masson and Diane Provencher Centre Hospitalier de L’université de Montréal
SKU: 161782
Product description: OV-3133(R) was established from ascites collected from patient 3133 at relapse after chemotherapy. It is part of a four-model same-patient series that also includes diagnosis-derived TOV3133D and TOV-3133G and the later relapse model OV-3133(R2). The series captures disease states sampled before and after multiple treatments, enabling longitudinal comparison of treatment-associated changes while controlling for patient background. OV-3133(R) was also the only one of the nine lines in the original study reported to form subcutaneous tumours in SCID mice.
Alternate name: OV-3133(R)
Gender: Female
CRISPR: No
Production details: Established from the cellular fraction of ascites collected by centrifugation. The cell lines were then maintained for 40 days in OSE with medium replaced weekly.
Additional notes: Patient 3133 received treatment of paclitaxel and carboplatin 1-3 months after sugery and confirmation of ovarian cancer diagnosis. A second sugery 6 months after the first detectede infiltration of the tumor in part of the abdomen. Patient was treated with dxorubicin and later carboplatin and gemcitabine after no significant changes of CA-125 levels were seen. Later imaging presented an increase in tumor mass whereby she was given etoposide orally for eight days.
Cellosaurus ID: CVCL_9T15
Receptors of note: No
Related tools: 161899 ; 161781 ; 161783
Cat. #: 161782
Cancer types: Ovarian cancer
Research Fields: Cancer
Organism: Human
Tissue: Derived from ascites of patient diagnosed with high-grade serous ovarian cancer at specific time points at diagnosis and relapse
Gender: Female
Morphology: Formed tumors at subcutaneous sites in SCID mice. No expressionof the tumor suppressor p53 detected. Strong HER2 expression detected in protein extracts and observed in solid tissues by IHC. Spheroid formation: no
Growth properties: Adherent
Primary citation: Létourneau et al. 2012. BMC Cancer. (12): 379. PMID: 22931248
Shipping conditions: Dry Ice
Growth medium: OSE medium contains 10% FBS, 0.5ug/mL amphotericin B and 50 ug/mL gentamicin
Atmosphere: Hypoxic condition of 5%O2 and 5%CO2
References:

Tomas et al. 2023. J Ovarian Res. 11

16(1):70. PMID: 37038202 Vias et al. 2023. Elife. 11

12:e83867. PMID: 37166279 Jubelin et al. 2022. Cell Biosci. 11

12(1):155. PMID: 36089610 Canals et al. 2022. Front Oncol. 4

12:856424. PMID: 35600398

Related Tools for Cell lines

Cat. # Tool Name
161781 TOV-3133G cell line Key Info View Tool
161899 TOV3133D Key Info View Tool
161783 OV-3133(R2) cell line Key Info View Tool

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