MDA-MB-231 TetOn-3G MRCKBeta cell line

This cell line has been engineered to enable tetracycline inducible expression of the kinase MRCK?. MRCK? is a myotonic dystrophy kinase-related CDC42-binding kinase involved in regulating actin-myosin contractility and implicated in cancer metastasis. MRCK?, in conjunction with MRCKa, ROCK1 and ROCK2 kinases, initiates signalling events that lead to contractile force generation which powers cancer cell motility and invasion.

Contributors

Inventor Institute
Daniel Croft ; Justin Bower ; Heather Mckinnon Cancer Research UK, Glasgow: The Beatson Institute
SKU: 154149
Product description: This cell line has been engineered to enable tetracycline inducible expression of the kinase MRCK?. MRCK? is a myotonic dystrophy kinase-related CDC42-binding kinase involved in regulating actin-myosin contractility and implicated in cancer metastasis. MRCK?, in conjunction with MRCKa, ROCK1 and ROCK2 kinases, initiates signalling events that lead to contractile force generation which powers cancer cell motility and invasion. The cell line is a derivative of a human breast cancer cell line shown to reliably metastasize to clinically relevant sites (the lungs and lymph nodes) when implanted orthotopically in mice (i.e. when implanted in the breast pad of mice). The cell line expresses luciferase enabling detection of the location of the cells using bioluminescent imaging. Useful for the study of breast cancer cell cytoskeleton reorganisation and cell migration. This cell line is derived from a triple negative breast cancer (TNBC) meaning it does not express oestrogen, progesterone or HER2 receptors. Treatment with doxycycline induced MRCKÎ?‹Â› kinase domain expression and resulted in increased MLC phosphorylation. Clone demonstrated profound membrane blebbing following doxycycline treatment. Transgene expression did not appear to be ‘leaky’ in any clones tested.
Alternate name: Serine/threonine-protein kinase MRCK beta, CDC42-binding protein kinase beta, CDC42BP-beta, DMPK-like beta, Myotonic dystrophy kinase-related CDC42-binding kinase beta, MRCK beta, Myotonic dystrophy protein kinase-like beta
Conditional: Yes
Conditional description: MDA-MB-231 D3H2LN-Luc cell line that expresses tetracycline (Tet)-regulated transactivator Tet-On 3G regulated expression of MRCKÎ?‹Â›. Therefore MRCKÎ?‹Â› expression is induced in the presence of doxycycline.
Additional notes: Treatment with doxycycline induced MRCK? kinase domain expression and resulted in increased MLC phosphorylation. Clone demonstrated profound membrane blebbing following doxycycline treatment. Transgene expression did not appear to be ‘leaky’ in any clones tested. Tet-On System owned by TET Systems GmbH & Co. KG.
Parental cell line: MDA-MB-231 D3H2LN-Luc cell line
Disease: Cancer
Cat. #: 154149
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Organism: Human
Tissue: Breast
Model: Transgenic
Growth properties: Adherent cell line. Membrane blebbing/cytoskeleton reorganisation and migration induced in the presence of doxycycline.
Cancer Types In Detail: Triple negative breast (TNBC)
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: 10% FBS/MEM-EBSS/NEAA/NaPyr/Glutamine
Recommended controls: MDA-MB-231 D3H2LN-Luc parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Unbekandt et al. 2014. Cell Commun Signal. 12:54. PMID: 25288205.

A novel small-molecule MRCK inhibitor blocks cancer cell invasion.

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