MCF7/TAMR-4 Cell Line

A human tamoxifen-resistant breast cancer model used to study endocrine resistance, oestrogen receptor signalling, and hormone-directed therapies. Save 30% on this cell line this autumn. Use code ACA26CELL at checkout.

Contributors

Inventor Institute
Anne Lykkesfeldt Danish Cancer Society, Denmark
SKU: 152088
Product description: MCF7/TAMR-4 cell line is a stable tamoxifen-resistant subline. This cell line was produced from the parental cell line MCF7/S0.5, as a model cell system to study the effects of tamoxifen resistant cancer growth. MCF7/TAMR-4 cells are oestrogen receptor positive and progesterone receptor negative, and are growth inhibited by the pure antioestrogen fulvestrant. MCF7/TAMR-4 enables identification of new hormone therapies and greater understanding of the signalling pathways/methods behind tamoxifen resistance. Additionally, this line can aid in identifying new predictive markers for response to hormonal therapy.
Alternate name: MCF-7/TAMR-4 Cell Line
Gender: Female
Conditional: Yes
Production details: The parental cell line for the MCF7/TAMR-4 cells is MCF7/S0.5, which was adapted to grow at low serum concentration in order to study the effect of estradiol and tamoxifen. MCF7/TAMR-4 has been established from a clone of cells that survived long term treatment with 1 uM tamoxifen. Tamoxifen-resistant cells are passaged continuously in presence of 1 uM tamoxifen, which is lethal for the parental MCF7/S0.5 cell line.
Additional notes: MCF7/TAMR-4 cells are oestrogen receptor positive and progesterone receptor negative. MCF7/TAMR-4 cells are growth inhibited by the pure antiestrogen fulvestrant. The oestrogen receptor is a major driver of growth of MCF7/TAMR-4 cell, ref 1. Treatment targeting the Aurora kinase A restores sensitivity to tamoxifen treatment. The TAMR lines were established from the MCF7/S0.5 cell line, which was adapted to grow with 0.5% fetal calf serum in phenol red containing DMEM/F12 medium. Treatment wi…
Cellosaurus ID: CVCL_1D42
Parental cell line: MCF7 S0.5
Disease: Cancer
Cat. #: 152088
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Organism: Human
Tissue: Breast
Gender: Female
Model: Tumour line
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM:Ham’s F-12 phenol red-free medium containing 1% fetal bovine serum, 2 mM Glutamax and 6 ng/ml insulin. To maintain high-level resistance, medium was supplemented with Tamoxifen (1 uM).
Temperature: 37° C
Atmosphere: 5% CO2
Recommended controls: MCF7-S0.5 parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Joshi et al. 2016. Oncotarget. 7(35):57239-57253. PMID: 27528030.

Elias et al. 2015. Oncogene. 34(15):1919-1927. PMID: 24882577.

Thrane et al. 2015. Oncogene. 34:4199–4210. PMID: 25362855.

Thrane et al. 2013. Breast Cancer Res Treat. 139(1):71-80. PMID: 23609470.

Cutrupi et al. 2012. Oncogene. 31(40):4353-4361. PMID: 22249258.

Millour et al. 2010. Oncogene. 29(20):2983-2995. PMID: 20208560.

Pancholi et al. 2008. Endocr Relat Cancer. 15(4):985-1002. PMID: 18824559.

Sarwar et al. 2006. Endocr Relat Cancer. 13(3):851-861. PMID: 16954434.

Images

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MCF7 TAMR-4 Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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