MCF7/TAMR-1 Cell Line
Tamoxifen resistant cell line which can be used to discover alternative treatments for breast cancer and understand the signalling pathways involved in tamoxifen resistance.
Contributors
| Inventor | Institute |
|---|---|
| Anne Lykkesfeldt | Danish Cancer Society, Denmark |
| SKU: | 152089 |
|---|---|
| Product description: | Tamoxifen resistant cell line which can be used to discover alternative treatments for breast cancer and understand the signalling pathways involved in tamoxifen resistance. |
| Alternate name: | MCF-7/TAMR-1; MCF7/TAMR-1; MCF7 TAMR-1; MCF-7/TAM(R)-1; MCF7-TAMR; TAMR-1; TamR-1; TamR1; AL-1 |
| Gender: | Female |
| CRISPR: | No |
| Production details: | The parental cell line for the MCF7/TAMR-1 cells is MCF7/S0.5, which was adapted to grow at low serum concentration in order to study the effect of estradiol and tamoxifen. MCF7/TAMR-1 has been established from a clone of cells that survived long term treatment with 1 uM tamoxifen. The establishment of the MCF7/TAMR-1 cell line, originally named AL-1, was first described in Lykkesfeldt et al (1986). Tamoxifen-resistant cells are passaged continuously in presence of 1 uM tamoxifen, which is le… |
| Additional notes: | During the establishment process the treatment of MCF7/S0.5 cells with tamoxifen was started in passage 351. Few colonies of cells survived the treatment and after 28 days of tamoxifen treatment, tamoxifen was omitted from the medium for 22 days. After 19 passages without tamoxifen (passage 372) the cells underwent a second treatment with tamoxifen which initially reduced cell growth rate, but around 390-400 the growth rate of the tamoxifen resistant cell lines was close to the growth rate of… |
| Cellosaurus ID: | CVCL_M436 |
| Receptors of note: | Oestrogen receptor positive and progesterone receptor negative |
| Parental cell line: | MCF7/S0.5 Cell Line |
| Disease: | Cancer |
| Cat. #: | 152089 |
|---|---|
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Breast cancer |
| Research Fields: | Cancer; Drug development |
| Organism: | Human |
| Tissue: | Breast |
| Gender: | Female |
| Model: | Tumour line |
| Cancer Types In Detail: | Breast cancer;Tamoxifen resistant |
| Primary citation: | Lykkesfeldt et al. 1986. Br J Cancer. 53(1):29-35. PMID: 3947513 |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | Phenol red-free DMEM/F-12 containing 1% Fetal bovine serum, 2 mM Glutamax and 6 ng/ml Insulin. To maintain high-level resistance, the medium should be supplemented with 1 uM Tamoxifen |
| Temperature: | 37° C |
| Atmosphere: | 5% CO2 |
| Recommended controls: | MCF7-S0.5 parental line |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Lee et al. 2018. Autophagy. 14(5):812-824. PMID: 29130361 Martin et al. 2017. Nat Commun. 8(1):1865. PMID: 29192207 Guney Eskiler et al. 2016. Cell Biol Toxicol. :. PMID: 27585693 Joshi et al. 2016. Oncotarget. :. PMID: 27528030 Leclercq et al. 2016. J Recept Signal Transduct Res. :1-18. PMID: 27400858 Guest et al. 2016. PLoS One. 11(6):e0157397. PMID: 27308830 Alves et al. 2016. Clin Cancer Res. :. PMID: 27252418 Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755 Elias et a… |
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