MCF7/S0.5 Cell Line

A human ER-positive breast cancer model used to explore endocrine signalling, hormone response, and therapeutic development. Save 30% on this cell line this summer. Use code SUM26CELL at checkout.

Contributors

Inventor Institute
Anne Lykkesfeldt Danish Cancer Society
SKU: 152090
Product description: The MCF7/S0.5 human breast cancer cell line has been established to grow at low serum to reduce the oestrogen supply via serum and to enable oestrogen receptor-mediated growth inhibition with anti-oestrogens like tamoxifen. These cells are also useful for studies investigating the effects of oestrogens if cultured with 10% newborn calf serum, instead of 1% foetal calf serum, which has very low oestrogenic activity. This line expresses both oestrogen and progesterone receptors. Tamoxifen, fulvestrant, letrozole, anastrozole, and exemestane resistant cell lines have been derived from MCF7/S0.5 and so this line can be utilized as a parental sensitive control line.
Alternate name: MCF7/S0.5; MCF-7/S0.5; MCF7 S0.5
Gender: Female
CRISPR: No
Conditional: Yes
Production details: This MCF7 subline was established buy stepwise reduction in the serum concentration from 5% fetal calf serum to 0.5 % in growth medium consisting of DMEM: Ham’s F-12 medium (1:1) supplemented with 2mM glutamine and insulin (6ng/ml)
Cellosaurus ID: CVCL_1D47
Receptors of note: Oestrogen receptor and progesterone receptors
Parental cell line: MCF7
Disease: Cancer
Cat. #: 152090
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Organism: Human
Tissue: Breast
Gender: Female
Model: Tumour line
Morphology: Epithelial; Adherent polygonal epithelia
Growth properties: Adherent
Cancer Types In Detail: Breast cancer
Primary citation: Briand et al. 1984. Cancer Res. 44(3):1114-9. PMID: 6362856
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Phenol-red-free DMEM/F12 medium supplemented with 1% Fetal calf serum 2.5 mM Glutamax and 6 ng/ ml Insulin
Subculture routine: Split at 70-80% confluence: 1:3 to 1:6 (seeding at 2-4×104 cells/cm2) using 0.05% trypsin or trypsin or 0.05% trypsin/EDTA solution. Change the media every 2-3 days
Temperature: 37° C
Atmosphere: 5% CO2
Mycoplasma free: Yes
Biosafety level: 1
References:

Joshi et al. 2016. Oncotarget. :. PMID: 27528030

Alves et al. 2016. Clin Cancer Res. :. PMID: 27252418

Elias et al. 2015. Oncogene. 34:1919–1927. PMID: 24882577

Thomsen et al. 2015. Breast Cancer Res Treat. :. PMID: 26585578

Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755

Sonne-Hansen et al. 2005. J Steroid Biochem Mol Biol. 93(1):25-34. PMID: 15748829

Lykkesfeldt et al. 1995. Int J Cancer. 61(4):529-34. PMID: 7759159

Madsen et. al. 1995. Mol Cell Endocrinol 109(2):197-207. PMID: 7664983

Lykkesfeldt et al. 1994. Cancer Res. 54(6):1587-95. PMID: 8137264

Lykkesfeldt et al. 1989. Mol Cell Endocrinol. 62(2):287-96. PMID: 2744230

Lykkesfeldt et al. 1986. Br J Cancer. 53(1):29-35. PMID: 3947513

Briand et al. 1984. Cancer Res. 44(3):1114-9. PMID: 6362856.

Images

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MCF-7/S0.5 cells stained for oestrogen receptor expression

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