MCF7/TAMR-1 Cell Line

Tamoxifen resistant cell line which can be used to discover alternative treatments for breast cancer and understand the signalling pathways involved in tamoxifen resistance.

Contributors

Inventor Institute
Anne Lykkesfeldt Danish Cancer Society, Denmark
Cat. #: 152089
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Breast
Gender: Female
Model: Tumour line
Cancer Types In Detail: Breast cancer;Tamoxifen resistant
Primary citation: Lykkesfeldt et al. 1986. Br J Cancer. 53(1):29-35. PMID: 3947513
Alternate name: MCF-7/TAMR-1; MCF7/TAMR-1; MCF7 TAMR-1; MCF-7/TAM(R)-1; MCF7-TAMR; TAMR-1; TamR-1; TamR1; AL-1
Product description: Tamoxifen resistant cell line which can be used to discover alternative treatments for breast cancer and understand the signalling pathways involved in tamoxifen resistance.
Gender: Female
CRISPR: No
Production details: The parental cell line for the MCF7/TAMR-1 cells is MCF7/S0.5, which was adapted to grow at low serum concentration in order to study the effect of estradiol and tamoxifen. MCF7/TAMR-1 has been established from a clone of cells that survived long term treatment with 1 uM tamoxifen. The establishment of the MCF7/TAMR-1 cell line, originally named AL-1, was first described in Lykkesfeldt et al (1986). Tamoxifen-resistant cells are passaged continuously in presence of 1 uM tamoxifen, which is le…
Additional notes: During the establishment process the treatment of MCF7/S0.5 cells with tamoxifen was started in passage 351. Few colonies of cells survived the treatment and after 28 days of tamoxifen treatment, tamoxifen was omitted from the medium for 22 days. After 19 passages without tamoxifen (passage 372) the cells underwent a second treatment with tamoxifen which initially reduced cell growth rate, but around 390-400 the growth rate of the tamoxifen resistant cell lines was close to the growth rate of…
Cellosaurus ID: CVCL_M436
Receptors of note: Oestrogen receptor positive and progesterone receptor negative
Parental cell line: MCF7/S0.5 Cell Line
Disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Phenol red-free DMEM/F-12 containing 1% Fetal bovine serum, 2 mM Glutamax and 6 ng/ml Insulin. To maintain high-level resistance, the medium should be supplemented with 1 uM Tamoxifen
Temperature: 37° C
Atmosphere: 5% CO2
Recommended controls: MCF7-S0.5 parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Lee et al. 2018. Autophagy. 14(5):812-824. PMID: 29130361

Martin et al. 2017. Nat Commun. 8(1):1865. PMID: 29192207

Guney Eskiler et al. 2016. Cell Biol Toxicol. :. PMID: 27585693

Joshi et al. 2016. Oncotarget. :. PMID: 27528030

Leclercq et al. 2016. J Recept Signal Transduct Res. :1-18. PMID: 27400858

Guest et al. 2016. PLoS One. 11(6):e0157397. PMID: 27308830

Alves et al. 2016. Clin Cancer Res. :. PMID: 27252418

Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755

Elias et a…

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Establishment of MCF-7/TAMR-1 Cell line

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