Malme-3M Cell Line

Malme-3M is a malignant human melanoma cell line that displays fibroblast-like morphology and grows in mixed culture (adherent-suspension). This cell line has been shown to be dependent upon micropthalmia-associated transcription factor (MITF) activity for growth and survival. Malme-3M cells form tumours when injected into immunocompromised mice. These cells express mutant B-Raf (V600E) and wildtype N-Ras.

Contributors

Inventor Institute
Jorgen Fogh, Germain Trempe Memorial Sloan-Kettering Cancer Center (MSK)
Cat. #: 161910
Cancer types: Lung cancer
Organism: Human
Tissue: Skin
Gender: Male
Morphology: Mixed
Growth properties: Mixed: adherent and suspension
Cancer Types In Detail: Malignant Melanoma
Primary citation: Fogh J et al. 1977. Journal of the National Cancer Institute. 59: 221-226.PMID: 327080.
Product description: Malme-3M is a malignant human melanoma cell line that displays fibroblast-like morphology and grows in mixed culture (adherent-suspension). This cell line has been shown to be dependent upon micropthalmia-associated transcription factor (MITF) activity for growth and survival. Malme-3M cells form tumours when injected into immunocompromised mice. These cells express mutant B-Raf (V600E) and wildtype N-Ras.
Gender: Male
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions: Dry Ice
Growth medium: Iscove's Modified Dulbecco's Medium supplemened with 20% FBS
Temperature: 37° C
Atmosphere: 95% Air, 5% CO2
Storage medium: Culture medium 95%; DMSO, 5%
Biosafety level: 1
References:

Fogh J et al. 1977. Journal of the National Cancer Institute. 59: 221-226.PMID: 327080.

Xing F et al. 2012. Oncogene. 31: 446-457. PMID: 21725359.

Ma J et al. 2013. PLoS One. 8: e73261. PMID: 24015299.

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