HT-29 Cell Line

HT-29 is a widely used human colorectal adenocarcinoma cell line, exhibiting sensitivity to chemotherapeutic agents such as 5-fluorouracil (5FU) and oxaliplatin. These cells display phenotypic characteristics of mature intestinal cells, including enterocytes, making them a well-established in vitro model for studying food digestion and bioavailability. Additionally, HT-29 cells are used in drug discovery and microbiological […]

Contributors

Inventor Institute
Jorgen Fogh Memorial Sloan-Kettering Cancer Center (MSK)
Cat. #: 161929
Cancer types: Colorectal cancer
Tissue: Colon tissue
Gender: Female
Morphology: Epithelial
Growth properties: Adherent
Primary citation: Fogh et al. 1977. Journal of the National Cancer Institute. 59.1.221. PMID: 327080.
Alternate name: HT29; HT 29
Product description: HT-29 is a widely used human colorectal adenocarcinoma cell line, exhibiting sensitivity to chemotherapeutic agents such as 5-fluorouracil (5FU) and oxaliplatin. These cells display phenotypic characteristics of mature intestinal cells, including enterocytes, making them a well-established in vitro model for studying food digestion and bioavailability. Additionally, HT-29 cells are used in drug discovery and microbiological research to investigate probiotics and bacteriophage therapies.
Gender: Female
Disease: Adenocarcinoma;Colorectal Cancer
Products or characteristics of interest: Karyotype: hypertriploid, modal number = 71; range = 68 to 72. Isoenzymes: AK-1, 1; ES-D, 1-2; G6PD, B; GLO-I, 2; PGM1, 1-2; PGM3, 2
Shipping conditions: Dry Ice
Growth medium: McCoy's 5a Medium Modified with fetal bovine serum to a final concentration of 10%.
Temperature: 37° C
Atmosphere: 5% CO2 in air
Storage medium: Complete growth medium supplemented with 5% DMSO
Biosafety level: 1
References:

Nautiyal et al. 2011. Journal of molecular signaling. 6:7. PMID: 21774804.

Cohen et al. 1999. Journal of cell science. 112.16.2657. PMID: 10413674.

Images

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HT-29 colorectal adenocarcinoma cell line (Cat. #: 161929) shown at low magnification. Image courtesy of Memorial Sloan Kettering Cancer Center.
HT-29 colorectal adenocarcinoma cell line (Cat. #: 161929) shown at high magnification. Image courtesy of Memorial Sloan Kettering Cancer Center.

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