HeLa Flp-In SLC7A5+SLC7A5 3’UTR

To identify genes whose expression is controlled by YBX3

Contributors

Inventor Institute
Matthias Hentze European Molecular Biology Laboratory (EMBL)
SKU: 160840
Product description: To identify genes whose expression is controlled by YBX3
Production details: Adherent HeLa cells were grown at 37Â?‚°C and 5% CO2 in DMEM, supplemented with 10% FBS Gold (PAA), 1% Penicillin/Streptomycin (Sigma) and 1% L-glutamine (GIBCO). Cells were grown until 70%â?‚€?‚“80% confluent, trypsinized, counted (Biorad TC20) and 200,000 cells were seeded per well in a 6-well dish for reverse transfection of siRNAs following the manufacturerâ?‚€?‚™s recommendations (Lipofectamine RNAiMax, Invitrogen, cat# 137780755). YBX3 and control siRNAs were obtained from…
Parental cell line: HeLa FLP-IN T-REx
Cat. #: 160840
Unit size: 1×10^6 cells / vial
Research Fields: Metabolism
Model: Knock-Out
Format: Frozen
Shipping conditions: Dry Ice
Growth medium: Adherent HeLa cells were grown at 37?‚°C and 5% CO2 in DMEM, supplemented with 10% FBS Gold (PAA), 1% Penicillin/Streptomycin (Sigma) and 1% L-glutamine (GIBCO) Grown in medium containing blasticidine (5mg/ml) and hygromycin (200mg/ml). Cells were grown until 70%â?‚€?‚“80% confluent, trypsinized, counted (Biorad TC20) and 200,000 cells were seeded per well in a 6-well dish for reverse transfection of siRNAs following the manufacturerâ?‚€?‚™s recommendations (Lipofectamine RNAiMax,…
References:

The RNA-Binding Protein YBX3 Controls Amino Acid Levels by Regulating SLC mRNA Abundance

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