HCT 116 p300 KO Rescue Cell Line

This p300 rescue cell line was generated from p300 KO Clone F5.

Contributors

Inventor Institute
Carlos Caldas Cancer Research UK, Cambridge Institute
Cat. #: 153186
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Colorectal cancer
Research Fields: Cancer;Cell biology;Drug development
Organism: Human
Tissue: Colon
Model: Knock-In
Growth properties: Adhesion properties; invasion; migration
Cancer Types In Detail: Carcinoma
Product description: This p300 rescue cell line was generated from p300 KO Clone F5.The HCT 116 p300 KO Cell Line is a tool for the in vitro study of p300 in colon carcinoma, and specifically of its role in p53-dependent apoptosis, cellular adhesion and migration. Cell lines available are p300 WT (HCT116), p300 KO Clone D10 (HCT116), p300 KO Clone F5 (HCT116), p300 KO Clone F2 (HCT116). Experiments conducted should be using at least 2 of the 3 KO cell lines. The best 2 KO clones are D10 and F5. Anyone requesting the cell line should use the parental cell line that the KOs were generated from. The p300 KO lines demonstrate an aggressive cancer phenotype in vitro, with loss of cell-cell adhesion, defects in cell-matrix adhesion, and increased migration through collagen matrices. p300 is a transcriptional cofactor involved in regulating multiple cellular processes including cell cycle regulation, proliferation, differentiation, apoptosis, DNA damage repair and adhesion properties. Somatic inactivating mutations of p300 are associated with several cancers including breast, colorectal and gastric cancers.
Conditional: Yes
Additional notes: CBP was not manipulated in this cell line
Cellosaurus ID: CVCL_HG30
Parental cell line: HCT 116
Disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: McCoy's 5A medium + 10% FCS
Recommended controls: HCT 116 parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Wallace et al. 2015. PLoS Pathog. 11(3):e1004687. PMID: 25803638.

Lyer et al. 2007. Oncogene. 26(1):21-29. PMID: 16878158.

Bundy et al. 2006. Cancer Res. 66(15):7606-7614. PMID: 16885360.

Krubasik et al. 2006. Br J Cancer. 94(9):1326-1332. PMID: 16622451.

Lyer et al. 2004. Proc Natl Acad Sci U S A. 101(19):7386-7391. PMID: 15123817.

Images

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HCT 116 p300 KO Rescue Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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