HCT 116 BRCA2 -/- 46 Cell Line

HCT116 BRCA2 -/- [46] human colorectal carcinoma cell line generated to study the role of BRCA2 in DNA repair and a range of cancers.

Contributors

Inventor Institute
Carlos Caldas Cancer Research UK Cambridge Institute
Cat. #: 153531
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Colorectal cancer;Breast cancer
Research Fields: Cancer;Drug development;Genetics
Organism: Human
Tissue: Colon
Model: Knock-Out
Cancer Types In Detail: Colorectal carcinoma
Primary citation: Xu et al. 2014. J Pathol. 234(3):386-97. PMID: 25043256
Alternate name: HCT 116 BRCA2(-/-) clone 46; HCT 116 BRCA2(-/-)
Product description: HCT116 BRCA2 -/- [46] human colorectal carcinoma cell line generated to study the role of BRCA2 in DNA repair and a range of cancers.
CRISPR: No
Conditional: No
Production details: Targeted disruption of the human BRCA2 locus in HCT116 cells by homologous recombination. The gene targeting construct was generated by using a recombinant adeno-associated virus (rAAV) system. The deletion was confirmed by Southern Blot and Western Blot using antibodies against the C-terminus of the BRCA2 protein. This cell line was noted as clone 46 by the originating laboratory
Additional notes: Clone 42 was generated at the same time as clone 46 cited in Xu et al. 2014. J Pathol. 234(3):386-97. PMID: 25043256. The originating laboratory have advised they observed no difference in culture phenotype between clone 42 and clone 46.
Cellosaurus ID: CVCL_VP26
Parental cell line: HCT 116 cell line
Model description: Targeted disruption of human BRCA2 by homologous recombination
Disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: McCoy's 5a medium modified, with 10% foetal bovine serum (FBS) and 2mM L-Glutamine
Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10⁴ cells/cm² using 0.05% trypsin/EDTA solution. Cells are cultured at 37°C, in 95% air and 5% CO₂, and passaged every 3 to 4 days.
Recommended controls: HCT 116 parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Xu et al. 2014. J Pathol. 234(3):386-97. PMID: 25043256

Issaeva et al. 2010. Cancer Res. 70(15):6268-76. PMID: 20631063

Images

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HCT 116 BRCA2 -/- 46 Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.

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