A2780ADR Cell Line

Premium cryopreserved A2780ADR cells resistant to adriamycin for the purpose of transfection and conducting research on ovarian cancer.

Contributors

Inventor Institute
Timothy Ward National Cancer Institute
Cat. #: 152707
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Organism: Human
Tissue: Ovary
Gender: Male
Model: Tumour line
Growth properties: Adherent
Cancer Types In Detail: Carcinoma;Carcinoma
Alternate name: A2780 ADR; A2780/ADR; A2780-ADR; A2780Adr; A2780ADR Cells; adriamycin-resistant cell line
Product description: Access an adriamycin-resistant derivative of the gold standard ovarian cancer line A2780. The A2780 series of ovarian cancer cell lines have been effective cancer research tools for over 20 years. A2780ADR is an adriamycin-resistant cell line that has been developed by exposure of adriamycin to the parent A2780 cell line (catalogue no. 152706). Adriamycin (doxorubicin) is frequently used as a therapeutic agent for cancer, by interfering with DNA replication. A2780ADR cells grow as a monolayer and in suspension in spinner cultures and are tumourigenic in immune deficient mice. To maintain resistance, adriamycin must be added to the culture medium. Key features of A2780ADR: (1) Cross-resistance to melphalan and vinblastine. (2) Useful in investigation of drug resistance mechanisms in cancer.
Gender: Male
CRISPR: Yes
Conditional: Yes
Production details: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5×1,000 to 2×10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Recommendation: culture cells without drug after resuscitation until growth has been fully established.
Additional notes: STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 11,14 D5S818: 11,12 D7S820: 10 THO1: 6 TPOX: 8,9 vWA: 15,16
Cellosaurus ID: CVCL_1941
Parental cell line: A2780
Disease: Cancer;Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS); treatment with 10E-7 M adriamycin at least once a week.
Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x10,000cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
Mycoplasma free: Yes
References:

Miller et al. RSC Adv. 2018. 8(69):39731-39734. PMID: 30713687.

Singh et al. Int J Pharm. 2015. 478(2):745-752. PMID: 25437111.

Beaufort et al. 2014. PLoS One. 9(9):e103988. PMID: 25230021.

Hamilton et al. 1984. Semin Oncol. 11(3):285-298. PMID: 6385258.

Images

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A2780ADR Cell Line. 24 hours post plating. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

Citations And Reviews

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