Cat. #153815
MSTI Fluorescence High-Throughput Drug Assay
Cat. #: 153815
Sub-type: Fluorescent Probe
Availability: Please enquire for quantities and pricing
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: University of Wisconsin-Milwaukee
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: MSTI Fluorescence High-Throughput Drug Assay
- Research fields: Drug development
- Molecular formula: C19H20NS
- Tool sub type: Fluorescent Probe
- Description: This novel fluorescence-based high throughput assay allows for the detection of thiol-reactive drug candidates that are likely to irreversibly interact with biological targets. These promiscuous inhibitors can be identified rapidly, in parallel, for small molecule screening libraries using 384 or 1536 well plate formats. Testing small molecules for their ability to modulate cysteine residues of proteins in the early stages of drug discovery is expected to increase efficiency and success of ev...
- Iupac: Acetyl-(E)-2-(4-mercaptostyryl)-1,3,3-trimethyl-3H-indol-1-ium
- Molecular weight: 294.1
- Solubility: DMSO, DMF, methanol, water (partially)
- Additional notes: âŞMore accurate â Less interference from molecules due to use of far red spectrum⪠Faster â Can be used for high throughput screening (1536-well plate format)⪠More versatile â Identifies both electrophilic and redox reactive compounds⪠Stable product â Use of acetylated precursor allows for storage of the assay probe and itsreliable generation in situ
Handling
- Storage conditions: -20° C, protect from light
References
- McCallum et al. 2013. J Biomol Screen. 18(6):705-13. PMID: 23446699.
- High-throughput identification of promiscuous inhibitors from screening libraries with the use of a thiol-containing fluorescent probe.
