Figure S5. Fluorescence emission spectrum of 2 at 500 nM in different solvents; ?max = 480; 482; 487; and 491 nm for acetone; MeOH; MeCN; and DMSO respectively. Fluorescence emissions were measured with ?ex = 350 (ex. slit 10; em. slit 20) nm on an Agilent Technologies Cary Eclipse Fluorescence Spectrometer.

Cat. #157848

AzuFluorTM 483-Bpin: Azulene-Based Fluorescent Probe for ROS/RNS small molecule (tool compound)

Cat. #: 157848

Sub-type: Fluorescent Probe

Availability: Please enquire for quantities and pricing

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

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Contributor

Inventor: Lloyd Murfin ; Tony James ; Simon Lewis

Institute: University of Bath

Tool Details

Handling

Target Details

References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

Tool name: AzuFluorTM 483-Bpin: Azulene-Based Fluorescent Probe for ROS/RNS small molecule (tool compound)
Alternate name: AzuFluor^TM 483-Bpin, Diethyl 2-amino-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)azulene-1,3- dicarboxylate
Research fields: Cancer
Molecular formula: C22H28BNO6
Tool sub type: Fluorescent Probe
Primary target: Reactive Oxygen and Nitrogen Species
Description: Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are important mediators in many physiological and pathological processes. The one-electron reduction of O2 in vivo leads to the formation of O2- (superoxide), which in turn undergoes disproportionation catalyzed by superoxide dismutase to give O2 and H2O2 (hydrogen peroxide). Another fate of O2- is to react with endogenously produced NO (nitric oxide) to form ONOO- (peroxynitrite) via a nonenzymatic process. Ordinarily, the flux of H2O2 is tightly regulated; aberrant H2O2 production or overexposure is implicated in the pathogenesis of many diseases such as cancer and neurodegenerative conditions. Similarly, while ONOO- has roles in signal transduction, its strongly oxidizing and nitrating properties mean it can react in an uncontrolled manner with various biomolecules. Elevated levels of ONOO- have been linked to cardiovascular, neurodegenerative, and inflammatory diseases as well as cancer. In view of this, there is significant need for tools and techniques to elucidate the roles of ONOO-, H2O2, and other ROS/RNS in biological systems.
Molecular weight: 412.2046
Solubility: DMSO, MeCN, Acetone, MeOH
Additional notes: pH: Fluorescence intensity greatest at pH 7-8. Optimal two-photon excitation wavelength Îťex = 800nm.

Handling

Purity: 412.2046 g/mol
Storage conditions: Ambient
Shipping conditions: Dry Ice

Target Details

Primary target: Reactive Oxygen and Nitrogen Species

References

Murfin et al. 2019. J Am Chem Soc. 141(49):19389-19396. PMID: 31773957.