#160593

TG-aMHC-STOP-CD63NanoLuc mouse

Cat. #160593

TG-aMHC-STOP-CD63NanoLuc mouse

Cat. #: 160593

Sub-type: Mouse

Availability: 6-8 weeks

Model: Knock-In

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Jiang Chang

Institute: Texas A&M University

Tool Details
Handling
Target Details
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Tool name: TG-aMHC-STOP-CD63NanoLuc mouse
  • Research fields: Cell biology;Cell signaling and signal transduction;Tissue-specific biology
  • Tool sub type: Mouse
  • Model: Knock-In
  • Conditional: Yes
  • Conditional description: CD63Nan
  • Description: This mouse models allows for researchers to track an visualize endogenous exosomes in real time. A NanoLuc reporter is fused to the exosome surface marker CD63 and expression is under control of a cardiomyocyte-specific promoter and conditional for tamoxifen.
  • Genetic background: In order to achieve a controllable exosome tagging, a dSTOP sequence flanked by two loxP sites was introduced upstream of CD63NanoLuc, and the expression cassette was driven by cardiomyocyte-specific Îą-MHC promoter (Fig). Linearized DNA containing ÎąMHC-STOP-CD63NanoLuc cassette was used for pronuclear microinjection to generate the founder of the transgenic strain.
  • Phenotype: Please see linked reference that details the generation and characterization of this mouse model.
  • Strain: C57BL/6
  • Additional notes: Please see linked reference that details the generation and characterization of this mouse model.

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Exosomes expressing CD63

References

  • Luo et al. 2020. Commun Biol. 3(1):114. PMID: 32157172.