TG-aMHC-STOP-CD63NanoLuc mouse

This mouse models allows for researchers to track an visualize endogenous exosomes in real time. A NanoLuc reporter is fused to the exosome surface marker CD63 and expression is under control of a cardiomyocyte-specific promoter and conditional for tamoxifen.

Contributors

Inventor Institute
Jiang Chang Texas A&M University
Cat. #: 160593
Research Fields: Cell biology;Cell signaling and signal transduction;Tissue-specific biology
Target: Exosomes expressing CD63
Model: Knock-In
Strain: C57BL/6
Phenotype: Please see linked reference that details the generation and characterization of this mouse model.
Product description: This mouse models allows for researchers to track an visualize endogenous exosomes in real time. A NanoLuc reporter is fused to the exosome surface marker CD63 and expression is under control of a cardiomyocyte-specific promoter and conditional for tamoxifen.
Conditional: Yes
Conditional description: CD63Nan
Genetic background: In order to achieve a controllable exosome tagging, a dSTOP sequence flanked by two loxP sites was introduced upstream of CD63NanoLuc, and the expression cassette was driven by cardiomyocyte-specific Îą-MHC promoter (Fig). Linearized DNA containing ÎąMHC-STOP-CD63NanoLuc cassette was used for pronuclear microinjection to generate the founder of the transgenic strain.
Additional notes: Please see linked reference that details the generation and characterization of this mouse model.
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Luo et al. 2020. Commun Biol. 3(1):114. PMID: 32157172.

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