Cat. #160593
TG-aMHC-STOP-CD63NanoLuc mouse
Cat. #: 160593
Sub-type: Mouse
Availability: 8-10 weeks
Model: Knock-In
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Jiang Chang
Institute: Texas A&M University
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: TG-aMHC-STOP-CD63NanoLuc mouse
- Research fields: Cell biology;Cell signaling and signal transduction;Tissue-specific biology
- Tool sub type: Mouse
- Model: Knock-In
- Conditional: Yes
- Conditional description: CD63Nan
- Description: This mouse models allows for researchers to track an visualize endogenous exosomes in real time. A NanoLuc reporter is fused to the exosome surface marker CD63 and expression is under control of a cardiomyocyte-specific promoter and conditional for tamoxifen.
- Genetic background: In order to achieve a controllable exosome tagging, a dSTOP sequence flanked by two loxP sites was introduced upstream of CD63NanoLuc, and the expression cassette was driven by cardiomyocyte-specific Îą-MHC promoter (Fig). Linearized DNA containing ÎąMHC-STOP-CD63NanoLuc cassette was used for pronuclear microinjection to generate the founder of the transgenic strain.
- Phenotype: Please see linked reference that details the generation and characterization of this mouse model.
- Strain: C57BL/6
- Additional notes: Please see linked reference that details the generation and characterization of this mouse model.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Exosomes expressing CD63
References
- Luo et al. 2020. Commun Biol. 3(1):114. PMID: 32157172.
