Spag17/Tomato-EGFP/CMV-Cre mouse

Knockout mouse model for the global deletion of the Spag17 gene linked to a Tomato-EGFP system for cell linage tracing

Contributors

Inventor Institute
Maria E Teves Ph.D. Virginia Commonwealth University
Cat. #: 161685
Research Fields: Cell biology;Organ fibrosis;Primary cilia dyskinesia;Skeletal dysplasia
disease: Development
Model: Knock-out
Zygosity: Homozygous
Strain: C57BL/6J wild-type females, 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J, and CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice
Phenotype: Multi-organ fibrosis
Primary citation: Teves et al. Am J Respir Cell Mol Biol. 48(6):765-72. PMID: 23418344
Product description: This is a knockout mouse model for the global deletion of the Spag17 gene linked to a Tomato-EGFP system for cell linage tracing. This mouse model has the potential to generate a knockout for Spag17 when crossed with a Cre mouse. Cells will express an EGFP fluorophore for in vivo tracking if they are deficient in Spag17 and presents an avanue for studying primary cili dyskinesia, development, skeletal dysplasia, primary cilia disfunction, aging, infertility, and multi-organ fibrosis
CRISPR: No
Genetic background: Spag17+/flox mice were mated with CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice to generate Spag17+/?;CMV-Cre mice. Male and female Spag17+/?;CMV-Cre mice were mated, resulting in Mendelian inheritance for Spag17?/?;CMV-Cre mice demonstrating a mixed background of C57BL/6J–129S4/SvJ
Production details: Embryonic stem cells with conditional potential were used to generate chimeric mice which were crossed to C57BL/6J wild-type females. Resulting mice were crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J mice to remove the Neo cassette. The Spag17+/flox mice were used for mating with CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice to generate Spag17+/?;CMV-Cre mice. Male and female Spag17+/?;CMV-Cre mice were mated, resulting in Mendelian inheritance for Spag17?/?;CMV-Cre mice. Afterwards, mice dem…
References:

Teves et al. Am J Respir Cell Mol Biol. 48(6):765-72. PMID: 23418344

Teves et al. PLoS One 10(5) PMID: 26017218

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