#161680

SAR-22-019F mouse line

Cat. #161680

SAR-22-019F mouse line

Cat. #: 161680

Disease: Non-alcoholic fatty liver disease (NAFLD)

Model: Transgenic

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Contributor

Inventor: Devanand Sarkar

Institute: Virginia Commonwealth University

Primary Citation: Manna et al. Hepatology doi: 10.1002/hep32797 PMID: 36120720

Tool Details
Application Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: SAR-22-019F mouse line
  • Cancer: Carcinoma
  • Research fields: Cancer
  • Disease: Non-alcoholic fatty liver disease (NAFLD)
  • Model: Transgenic
  • Crispr: No
  • Description: Transgenic mouse with hepatocyte-specific overexpression of MDA-9 (Alb/MDA-9). Alb/MDA-9 allows analysis of the role of MDA-9 in HCC development and progression. Alb/MDA-9 allows analysis of the molecular mechanism by which MDA-9 promotes HCC. This mouse line will also be useful in analyzing the role of MDA-9 in other liver diseases such as fibrosis. Findings identify a novel role of MDA-9/SDCBP in hepatocarcinogenesis thereby establishing the rationale for using MDA-9 inhibitors for the...
  • Application: qPCR, western blotting, IHC, and single-cell RNA-seq
  • Genetic background: This Alb/MDA-9 transgenic mouse line in B6CBAF1 background was generated by directing the expression of C-terminal HA-tagged human MDA-9/SDCBP under an upstream enhancer region (–10400 to –8500) fused to the 335-bp core region of mouse albumin promoter.
  • Phenotype: Higher tumor burden in livers , increased levels of serum liver enzymes, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in Alb/MDA-9 mice after 32 weeks
  • Strain: B6CBAF1
  • Production details: This Alb/MDA-9 transgenic mouse line in B6CBAF1 background was generated by directing the expression of C-terminal HA-tagged human MDA-9/SDCBP under an upstream enhancer region (–10400 to –8500) fused to the 335-bp core region of mouse albumin promoter.

Application Details

  • Application: qPCR, western blotting, IHC, and single-cell RNA-seq

References

  • Manna et al. Hepatology doi: 10.1002/hep32797 PMID: 36120720