Cat. #161680
SAR-22-019F mouse line
Cat. #: 161680
Disease: Non-alcoholic fatty liver disease (NAFLD)
Model: Transgenic
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Contributor
Inventor: Devanand Sarkar
Institute: Virginia Commonwealth University
Primary Citation: Manna et al. Hepatology doi: 10.1002/hep32797 PMID: 36120720
Tool Details
*FOR RESEARCH USE ONLY
- Tool name: SAR-22-019F mouse line
- Disease: Non-alcoholic fatty liver disease (NAFLD)
- Model: Transgenic
- Crispr: No
- Description: Transgenic mouse with hepatocyte-specific overexpression of MDA-9 (Alb/MDA-9). Alb/MDA-9 allows analysis of the role of MDA-9 in HCC development and progression.
Alb/MDA-9 allows analysis of the molecular mechanism by which MDA-9 promotes HCC. This mouse line will also be useful in analyzing the role of MDA-9 in other liver diseases such as fibrosis. Findings identify a novel role of MDA-9/SDCBP in hepatocarcinogenesis thereby establishing the rationale for using MDA-9 inhibitors for the... - Application: qPCR, western blotting, IHC, and single-cell RNA-seq
- Genetic background: This Alb/MDA-9 transgenic mouse line in B6CBAF1 background was generated by directing the expression of C-terminal HA-tagged human MDA-9/SDCBP under an upstream enhancer region (–10400 to –8500) fused to the 335-bp core region of mouse albumin promoter.
- Phenotype: Higher tumor burden in livers , increased levels of serum liver enzymes, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in Alb/MDA-9 mice after 32 weeks
- Strain: B6CBAF1
- Production details: This Alb/MDA-9 transgenic mouse line in B6CBAF1 background was generated by directing the expression of C-terminal HA-tagged human MDA-9/SDCBP under an upstream enhancer region (–10400 to –8500) fused to the 335-bp core region of mouse albumin promoter.
Application Details
- Application: qPCR, western blotting, IHC, and single-cell RNA-seq
References
- Manna et al. Hepatology doi: 10.1002/hep32797 PMID: 36120720
