Conditional knockout of Jag1; in vivo studies of tissue-specific Jag1 knockout and Notch signalling
| Inventor | Institute |
|---|---|
| Julian Lewis | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| SKU: | 151482 |
|---|---|
| Product description: | Conditional knockout of Jag1; in vivo studies of tissue-specific Jag1 knockout and Notch signalling |
| Conditional: | Yes |
| Conditional description: | Jag1 gene with loxP sites flanking exons 4 & 5 enabling conditional Cre-mediate knockout. |
| Production details: | A Jag1 targeting vector, containing a loxP flanked resistance cassette before exon 4 and a loxP site inserted after exon 5, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were transfected with a Cre expression vector to excise the resistance cassette, and injected into a C57BL6 blastocyst. Chimeric offspring were mated on a C57BL6 background. |
| Cat. #: | 151482 |
|---|---|
| Research Fields: | Cell signaling and signal transduction; Genetics; Neurobiology; Tissue-specific biology |
| Target: | Jagged1 (Jag1) |
| Disease: | Cancer |
| Model: | Knock-Out |
| Zygosity: | Heterozygous |
| Shipping conditions: | Embryo/Spermatoza- Dry Ice |
|---|
| References: |
Brooker et al. 2006. Development. 133(7):1277-86. PMID: 16495313. Notch ligands with contrasting functions: Jagged1 and Delta1 in the mouse inner ear. |
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