In vivo study of GSTP1/2 (regulator of protein synthesis/degradation) knockout in tumourigenesis, drug metabolism and toxicity; disease model for drug metabolism and toxicity; tumourigenesis model (skin, lung) (chemically induced)
| Inventor | Institute |
|---|---|
| Roland Wolf ; Colin Henderson | University of Dundee |
| SKU: | 151443 |
|---|---|
| Product description: | In vivo study of GSTP1/2 (regulator of protein synthesis/degradation) knockout in tumourigenesis, drug metabolism and toxicity; disease model for drug metabolism and toxicity; tumourigenesis model (skin, lung) (chemically induced) |
| Conditional: | No |
| Genetic background: | A Gstp1/2 targeting vector, replacing all of the Gstp1 gene and exons 6 and 7 of the Gstp2 gene with a resistance cassette, was transfected into E14Tg2a.IV ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into blastocysts. Chimeric mice were mated with MF1 mice to generate mice heterozygous for GSTP1/2 knockout. Heterozygous mice were interbred to generate mice homozygous for GSTP1/2 knockout. |
| Production details: | A Gstp1/2 targeting vector, replacing all of the Gstp1 gene and exons 6 and 7 of the Gstp2 gene with a resistance cassette, was transfected into E14Tg2a.IV ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into blastocysts. Chimeric mice were mated with MF1 mice to generate mice heterozygous for GSTP1/2 knockout. Heterozygous mice were interbred to generate mice homozygous for GSTP1/2 knockout. |
| Cat. #: | 151443 |
|---|---|
| Cancer types: | Lung cancer |
| Research Fields: | Cancer; Genetics |
| Target: | Glutathione Transferase P1 and P2 (GSTP1, GSTP2) |
| Disease: | Cancer |
| Model: | Knock-Out |
| Zygosity: | Homozygous |
| Phenotype: | Tumourigenesis (lung, skin); drug metabolism; drug toxicity |
| Primary citation: | Henderson et al. 1998. Proc Natl Acad Sci U S A. 95(9):5275-80. PMID: 9560266 |
| Shipping conditions: | Embryo/Spermatoza- Dry Ice |
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| References: |
Zhou et al. 2008. Am J Respir Crit Care Med. 178(12):1202-10. PMID: 18787219 Elsby et al. 2003. J Biol Chem. 278(25):22243-9. PMID: 12646564 Rosario et al. 2000. Mol Pharmacol. 58(1):167-74. PMID: 10860939 Henderson et al. 1998. Proc Natl Acad Sci U S A. 95(9):5275-80. PMID: 9560266 |
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