Cat. #154093
p53Lck-TLX1 Mouse
Cat. #: 154093
Sub-type: Mouse
Availability: 8-10 weeks
Disease: T cell acute lymphoblastic leukemia
Model: Transgenic
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Adolfo Ferrando
Institute: The Trustees of Columbia University in the City of New York
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: p53Lck-TLX1 Mouse
- Alternate name: T Cell Leukemia Homeobox 1, Homeo Box-11, Proto-Oncogene TCL-3, HOX11, TCL3
- Research fields: Cancer;Immunology
- Tool sub type: Mouse
- Disease: T cell acute lymphoblastic leukemia
- Model: Transgenic
- Model description: Transgenic mouse expressing human transcription factor Tlx1 in T cell progenitors under control of the LCK promoter
- Conditional: No
- Description: TLX1 is an orphan homeobox gene. It was originally discovered in association with 4-7% of cases of T cell acute lymphoblastic leukaemias harbouring a t(10;14)(q24;q11) cytogenetic abnormality. Research showed the presence of the TLX1 gene on chromosome 10q24 juxtaposed with the T cell receptor (TCR) delta regulatory elements on chromosome 10, resulting in aberrant over-expression of the full and intact TLX1 gene. Juxtaposition with the TCR alpha locus was also seen. Elevated TLX1 expression in leukemic blasts is also detected in the absence of a translocation in ~50% (37/76) of paediatric T-ALL cases, as well as rare cases of B-AL. Clinically, TLX1+ T-ALLs are associated with a genetic signature associated with maturation arrest at an early cortical thymocyte stage of T cell development. This model organism recapitulates many molecular features of human leukaemias induced by the TLX family of TF oncogenes.
- Genetic background: The human TLX1 cDNA was amplified by PCR using BamHI restriction site containing primers and cDNA of the human T-cell leukemia cell line ALL-SIL as template and was cloned in the pUC1017 vector, downstream of the mouse T-cell specific p56Lck proximal promoter
- Strain: C57BL/6
- Production details: The human TLX1 cDNA was amplified by PCR using BamHI restriction site containing primers and cDNA of the human T-cell leukemia cell line ALL-SIL as template and was cloned in the pUC1017 vector, downstream of the mouse T-cell specific p56Lck proximal promoter
- Additional notes: Transgenic mouse expressing human transcription factor Tlx1 in T cell progenitors under control of the LCK promoter
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: TLX1
References
- De Keersmaecker et al. 2010. Nat Med. 16(11):1321-7. PMID: 20972433.
- The TLX1 oncogene drives aneuploidy in T cell transformation.
