MEF2BD83V Mouse

Expression of the mutant MEF2BD38V allele was induced in germinal centre- derived B cells by crossing with C<U+03B3>1-Cre mice.

Contributors

Inventor Institute
Riccardo Dalla-Favera The Trustees of Columbia University in the City of New York
Cat. #: 154096
Research Fields: Cancer;Immunology
Target: MEF2B
disease: B-Cell Lymphoma
Model: Knock-In
Zygosity: Homozygous
Strain: C57BL/6
Alternate name: Myocyte Enhancer Factor 2B, Serum Response Factor-Like Protein 2, RSRFR2, XMEF2
Product description: Diffuse large B-Cell Lymphoma (DLBCL) and Follicular Lymphoma (FL) are the two most common forms of mature B cell lymphoid neoplams, accounting for over 50% of all diagnoses. Mutant MEF2B (MEF2BD83V) is the most common lymphoma associated oncogene. MEF2BD83V expression in mice leads to GC enlargement and lymphoma development, a phenotype that becomes fully penetrant in combination with BCL2 de-regulation, an event associated with human MEF2B mutations. Expression of the mutant MEF2BD38V allele was induced in germinal centre- derived B cells by crossing with C1-Cre mice.
Conditional description: Conditional Knockin mice that express mutant MEF2BD38V upon recombination by Cre-recombinase
Genetic background: The conditional Mef2bstopD83V allele was generated by introducing a single nucleotide change by site directed mutagenesis leading to the D83V amino-acid change in exon 3. The neomycin-resistance marker followed by a triple-polyA, flanked by two loxP sites was introduced upstream the exon 3 carrying the D83V mutation. Correctly targeted mouse ES cells were injected into blastocysts derived from C57BL/6 mice to generate chimeras.
Production details: The conditional Mef2bstopD83V allele was generated by introducing a single nucleotide change by site directed mutagenesis leading to the D83V amino-acid change in exon 3. The neomycin-resistance marker followed by a triple-polyA, flanked by two loxP sites was introduced upstream the exon 3 carrying the D83V mutation. Correctly targeted mouse ES cells were injected into blastocysts derived from C57BL/6 mice to generate chimeras.
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Brescia et al. 2018. Cancer Cell. 34(3):453-465.e9. PMID: 30205047.

MEF2B Instructs Germinal Center Development and Acts as an Oncogene in B Cell Lymphomagenesis.

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