Ln12 TCR Tg mice

The mouse model enables thymus selection and the study of emerging nave CD8+ T cells.Author publication:PMID: 29422902

Contributors

Inventor Institute
Thorbald Van Hall Leiden University and Leiden University Medical Center ; Stichting Oncode Institute
Cat. #: 157680
Research Fields: Cancer
Target: Ln12 TCR
Model: Transgenic
Zygosity: Unknown
Strain: C57BL/6
Product description: Tumor cells frequently escape from CD8+ T cell recognition by abrogating MHC-I antigen presentation. Deficiency in processing components, like the transporter associated with antigen processing (TAP), results in strongly decreased surface display of peptide/MHC-I complexes. A class of hidden self-antigens known as T cell epitopes associated with impaired peptide processing (TEIPP), which emerge on tumor cells with such processing defects can be investigated with this model. Using this mouse model, it is possible to investigate the generation of the TEIPP T cell repertoire specifically. These animals harbour rearranged receptors recognizing the Ln12 complex, recognizing a peptide in the context of the non-classical MHC class I molecule Qa-1b (encoded by the H2-T23 gene). The mouse model enables thymus selection and the study of emerging nave CD8+ T cells. Author publication: PMID: 29422902
Conditional: No
Conditional description: The expression of the TCR is driven by the CD2 promoter and not conditional
Genetic background: The Ln12 TCR-transgenic mouse strain was generated by transgenesis of the TCRa and TCR genes of the Ln12 T cell clone. The TCRa and TCR chains were separately cloned into pCRII-TOPO plasmid vectors using RT-PCR and sequenced. Expression of TCRs was performed by retroviral transduction of the TCR genes in C57BL/6 splenocytes using pMX vector. For transgenesis, the two chains were separately cloned into VA-hCD2 vectors and inserts were subsequently injected in C57BL/6 oocytes. (PMID: 29422902)
Production details: The Ln12 TCR-transgenic mouse strain was generated by transgenesis of the TCRι and TCRβ genes of the Ln12 T cell clone. The TCRι and TCRβ chains were separately cloned into pCRII-TOPO plasmid vectors using RT-PCR and sequenced. Expression of TCRs was performed by retroviral transduction of the TCR genes in C57BL/6 splenocytes using pMX vector. For transgenesis, the two chains were separately cloned into VA-hCD2 vectors and inserts were subsequently injected in C57BL/6 oocytes. (PMID: 29422902)
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Doorduijn et al. 2018. Front Immunol. 9:60. PMID: 29422902.

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