Cat. #151482

Jagged1 Floxed Mouse

Cat. #: 151482

Sub-type: Mouse

Availability: 8-10 weeks

Disease: Cancer

Model: Knock-Out

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Contributor

Inventor: Julian Lewis

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

Handling

Target Details

References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

Tool name: Jagged1 Floxed Mouse
Research fields: Cell signaling and signal transduction;Genetics;Neurobiology;Tissue-specific biology
Tool sub type: Mouse
Disease: Cancer
Model: Knock-Out
Conditional: Yes
Conditional description: Jag1 gene with loxP sites flanking exons 4 & 5 enabling conditional Cre-mediate knockout.
Description: Conditional knockout of Jag1; in vivo studies of tissue-specific Jag1 knockout and Notch signalling
Genetic background: A Jag1 targeting vector, containing a loxP flanked resistance cassette before exon 4 and a loxP site inserted after exon 5, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were transfected with a Cre expression vector to excise the resistance cassette, and injected into a C57BL6 blastocyst. Chimeric offspring were mated on a C57BL6 background.
Zygosity: Heterozygous
Production details: A Jag1 targeting vector, containing a loxP flanked resistance cassette before exon 4 and a loxP site inserted after exon 5, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were transfected with a Cre expression vector to excise the resistance cassette, and injected into a C57BL6 blastocyst. Chimeric offspring were mated on a C57BL6 background.

Handling

Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

Target: Jagged1 (Jag1)

References

Brooker et al. 2006. Development. 133(7):1277-86. PMID: 16495313.
Notch ligands with contrasting functions: Jagged1 and Delta1 in the mouse inner ear.