Cat. #151482
Jagged1 Floxed Mouse
Cat. #: 151482
Sub-type: Mouse
Availability: 6-8 weeks
Disease: Cancer
Model: Knock-Out
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Julian Lewis
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: Jagged1 Floxed Mouse
- Research fields: Cell signaling and signal transduction;Genetics;Neurobiology;Tissue-specific biology
- Tool sub type: Mouse
- Disease: Cancer
- Model: Knock-Out
- Conditional: Yes
- Conditional description: Jag1 gene with loxP sites flanking exons 4 & 5 enabling conditional Cre-mediate knockout.
- Description: Conditional knockout of Jag1; in vivo studies of tissue-specific Jag1 knockout and Notch signalling
- Genetic background: A Jag1 targeting vector, containing a loxP flanked resistance cassette before exon 4 and a loxP site inserted after exon 5, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were transfected with a Cre expression vector to excise the resistance cassette, and injected into a C57BL6 blastocyst. Chimeric offspring were mated on a C57BL6 background.
- Zygosity: Heterozygous
- Production details: A Jag1 targeting vector, containing a loxP flanked resistance cassette before exon 4 and a loxP site inserted after exon 5, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were transfected with a Cre expression vector to excise the resistance cassette, and injected into a C57BL6 blastocyst. Chimeric offspring were mated on a C57BL6 background.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Jagged1 (Jag1)
References
- Brooker et al. 2006. Development. 133(7):1277-86. PMID: 16495313.
- Notch ligands with contrasting functions: Jagged1 and Delta1 in the mouse inner ear.
