Hprt-CAG-LSL-KD2:ER Mouse

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Tool name: Hprt-CAG-LSL-KD2:ER Mouse
• Alternate name: Rho-associated protein kinase 2, Rho-associated coiled-coil-containing protein kinase 2, ROCK-II, Hypoxanthine phosphoribosyltransferase, HPRT, CAG, chicken actin gene, KD, Kinase dead,
• Research fields: Cancer;Cell biology;Genetics
• Tool sub type: Mouse
• Model: Transgenic
• Conditional: Yes
• Conditional description: Injection of tamoixfen activates excision of the LSL (loxP-stop-loxP) by Cre recombinase and enables expression of GFP:KD2 fusion protein
• Description: Rho associated coiled-coil containing protein kinases (ROCKs) exist in mammals, zebrafish, Xenopus, C. elegans and Drosophila. They are mainly involved in regulating the shape and movement of cells through acting on the cytoskeleton. Two mouse ROCK isoforms ROCK1 and ROCK2 have been identified. ROCK1 is mainly expressed in the lung, liver, spleen, kidney and testis and ROCK2 is distributed mostly in the brain and heart. ROCK2, an isoenzyme of ROCK1, is a serine/threonine kinase and regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and activation of the c-fos serum response element.
• Application: This strain can be crossed with mice expressing Cre recombinase under the transcriptional control of tissue specific promoters to restrict GFP:KD2:ER expression to tissues of choice.
• Genetic background: Kinase dead version has a single amino acid mutation (K125G) that blocks ATP-binding and kinase catalytic activity
• Zygosity: Heterozygous
• Strain: C57BL/6
• Production details: Kinase dead version has a single amino acid mutation (K125G) that blocks ATP-binding and kinase catalytic activity
• Additional notes: This strain can be crossed with mice expressing Cre recombinase under the transcriptional control of tissue specific promoters to restrict GFP:KD2:ER expression to tissues of choice.

Handling

• Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

• Target: Hprt gene-targeted Cre-inducible, tissue-specific expression of GFP:KD2 (kinase dead ROCK2)

Application Details

• Application: This strain can be crossed with mice expressing Cre recombinase under the transcriptional control of tissue specific promoters to restrict GFP:KD2:ER expression to tissues of choice.

Related Tools

• Related tools: Hprt-CAG-LSL-ROCK2:ER Mouse

References

• Tissue-selective expression of a conditionally-active ROCK2-estrogen receptor fusion protein.
• Samuel et al. 2016. Genesis. :. PMID: 27775859.