FH1 KO Mouse

the mouse may be useful for testing therapeutic interventions that target angiogenesis and HIF-prolyl hydroxylation.

Contributors

Inventor Institute
Ian Tomlinson Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151657
Research Fields: Cancer;Cell signaling and signal transduction;Genetics;Metabolism
Target: Fumarate Hydratase 1
Disease: Renal cyst
Model: Knock-Out
Phenotype: Fh1-/- mice died in early embryogenesis Fh1 fl/fl with Ksp1.3/Cre are healthy until ~8 months when polyuric renal failure occurs. Postmortem shows macroscopic renal cysts.
Product description: Germline mutations in the fumarate hydratase tumour suppressor gene predispose to leiomyomatosis, renal cysts, and renal cell cancer (HLRCC). Fh1-deficient mice develop renal cysts that have an activated hypoxia pathway, with overexpression of Hif1alpha and Hif2alpha. Hif targets, such as Glut1 and Vegf were also upregulated. Therefore the mouse may be useful for testing therapeutic interventions that target angiogenesis and HIF-prolyl hydroxylation.
Conditional: Yes
Conditional description: LoxP flanking exons 2 and 3 of Fh1
Genetic background: Following generation of the targeting construct, linearization, and electroporation into 129Sv/J ES cells, stable integrants were selected in 0.2 mg/ml Geneticin G418 medium. Homologous recombinants were identified initially by PCR and subsequently by Southern blot analysis. Targeted ES cells were injected into C57/BL6 blastocysts. ES cell clones were screened for 50 and 30 site-specific genomic integration of the targeting construct using a PCR-based assay and LATaq (Takara). PCR genotypes to assay Flp- and Cre-mediated recombination, and genotyping of eFlp and Cre expression mice were carriedout using standard conditions. For the genotyping of Fh1 to distinguish between wild-type, null, and floxed alleles, a common forward primer (50-GCTCAGTCACCCATCCAAAT-30 ) and differential reverse primers (50-ACCCTGCTAGGTGTCACCAC-30 and 50-CCTGGCACTGCAGACTACAA-30 ) were used
Production details: Following generation of the targeting construct, linearization, and electroporation into 129Sv/J ES cells, stable integrants were selected in 0.2 mg/ml Geneticin G418 medium. Homologous recombinants were identified initially by PCR and subsequently by Southern blot analysis. Targeted ES cells were injected into C57/BL6 blastocysts. ES cell clones were screened for 50 and 30 site-specific genomic integration of the targeting construct using a PCR-based assay and LATaq (Takara). PCR genotypes to assay Flp- and Cre-mediated recombination, and genotyping of eFlp and Cre expression mice were carriedout using standard conditions. For the genotyping of Fh1 to distinguish between wild-type, null, and floxed alleles, a common forward primer (50-GCTCAGTCACCCATCCAAAT-30 ) and differential reverse primers (50-ACCCTGCTAGGTGTCACCAC-30 and 50-CCTGGCACTGCAGACTACAA-30 ) were used
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Bardella et al. 2011. J Pathol. 225(1):4-11. PMID: 21630274.

Aberrant succination of proteins in fumarate hydratase-deficient mice and HLRCC patients is a robust biomarker of mutation status.

Pollard et al. 2007. Cancer Cell. 11(4):311-9. PMID: 17418408.

Targeted inactivation of fh1 causes proliferative renal cyst development and activation of the hypoxia pathway.

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