Double Floxed Fbxw7/FoxM1

FoxM1 is plays a key role in the cell cycle as endogenous expression peaks at the S and G2/M phases, crucial for mitotic division

Contributors

Inventor Institute
Abdolrahman Shams-Nateri University of Nottingham
Cat. #: 153594
Cancer types: Digestive / Gastrointestinal cancer
Research Fields: Cancer;Drug development;Metabolism
Target: Fbxw7 and foxM1
Model: Conditional KO
Zygosity: Homozygous
Strain: C57BL/6
Alternate name: F-box/WD repeat–containing protein 7, CDC4, Sel1, Ago, Fbw7, SCF, Skp1/Cullin/F-box protein, E3 ubiquitin ligase complex,FBW6, FBX3, FBXO3, FBXW6, SEL-1, hAgo, hCdc4, FOXM1, FKHL16, FOXM1B, HFH-11, HFH11, HNF-3, INS-1, MPHOSPH2, MPP-2, MPP2, PIG29, TGT3, TRIDENT, forkhead box M1
Product description: FoxM1 is plays a key role in the cell cycle as endogenous expression peaks at the S and G2/M phases, crucial for mitotic division. Recent studies have shown that FOXM1 regulates expression of a large array of G2/M-specific genes which play a crucial role in maintenance of genomic stability and chromosomal segregation. There are 3 known isoforms of FoxM1, A, B & C and it is a known human proto-oncogene, involved in basal cell carcinoma. Fbxw7 is a member of the F-box protein family, characterised by a motif of approximately 40 amino acids called the F-box. These proteins constitute one of the four subunits of ubiquitin protein ligase complex called SKP1-cullin-F-box and function in phosphorylation-dependent ubiquitination. Fbxw7 specifically protein contains 7 tandem solenoid protein domains also know as WD40 repeats. Fbwx7 binds directly to cyclin E and probably targets it for ubiquitin-mediated degradation.
Conditional: Yes
Conditional description: Requires expression of Cre recombinase to mediate excision of the lox P sites generating non-functional Fbxw7 and foxM1 proteins
Production details: This model was created by interbreeding the floxed-Fbxw7 mouse with a floxed-FoxM1 mouse (for floxed-FoxM1 mouse see references).
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Yoshida et al. 2007. Gastroenterology. 132(4):1420-31. PMID: 17408638.

The forkhead box M1 transcription factor contributes to the development and growth of mouse colorectal cancer.

Wang et al. 2002. Proc Natl Acad Sci U S A. 99(26):16881-6. PMID: 12482952.

The Forkhead Box m1b transcription factor is essential for hepatocyte DNA replication and mitosis during mouse liver regeneration.

Related Tools for Mouse models

Cat. # Tool Name
151443 GSTP1GSTP2 KO Mouse Key Info View Tool
151475 Raf KI D486A Mouse Key Info View Tool
151476 Raf-/- KO Mouse Key Info View Tool
151520 CDH5(PAC)CreERT2 Mouse Key Info View Tool
151554 SAP1 Mouse Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.