Wilms’ tumour 17.94 Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Wilms’ tumour 17.94 Cell Line
• Research fields: Cancer
• Organism: Human
• Tissue: Kidney
• Growth properties: When receiving a new vial, 17.94 cells are best started in a small vessel (for example a 12.5cm2 flask). This cell line tends to grow in clumps.
• Model: Tumour line
• Conditional: No
• Description: Wilms´ tumour 17.94 Cell Line is an anaplastic Wilms´ tumour (WT) cell line, which expressed NCAM, SALL1, and phenotypic features expected of metanephric blastema-derived cells. In Brown et al., 2005 treatment of 17.94 cells with 12-O-Tetradecanoylphorbol 13-acetate caused morphological changes, which led to reduced NCAM and SALL1 expression, but expression of vimentin was maintained. Wilms´ tumour 17.94 Cell Line contains a TP53 mutation but lacks mutations in WT1, WTX, or CTNNB1, other genes involved in WT pathogenesis. The cells demonstrate no loss of heterozygosity at 7p, 11p, or 16q although DNA hypermethylation was detected at several loci, including the H19 differentially methylated region and at the PCDH@ gene clusters at 5q31.
• Production details: Established from the nephrectomy specimen of a large lobulated Wilms´ tumour of a 4-year-old girl; cells were described to express NCAM, SALL1, and CITED1, and to contain a TP53 mutation. Freeze in 70% medium, 20% FBS, 10% DMSO
• Biosafety level: 1
• Cellosaurus id: CVCL_D704

Handling

• Format: Frozen
• Growth medium: 80% Dulbecco's MEM + 20% h.i. FBS at 37C with 5% CO2. Seed out at ca. 0.5-1 x 10^6 cells/25 cm2 flask; split semi-confluent culture 1:2 once a week using TrypLE Express.
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Storage medium: 70% medium, 20% FBS, 10% DMSO
• Mycoplasma free: Yes

References

• Brown et al. 2012. Cancer Genet. 205(6):319-326. PMID: 22749038.