Cat. #161126
UOL-Hep-CYB2 cell line
Cat. #: 161126
Availability: 8-10 weeks
Organism: Human
Tissue: Liver
Disease: Mitochondrial Subhaplogroup : H1c3
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Amy Chadwick
Institute: University of Liverpool, Molecular and Clinical Pharmacology
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: UOL-Hep-CYB2 cell line
- Research fields: Drug development
- Parental cell: HepG2
- Organism: Human
- Tissue: Liver
- Disease: Mitochondrial Subhaplogroup : H1c3
- Growth properties: Adherent
- Model description: Cybrid Cell Line
- Description: This cell line consists of HepG2 cybrid cell lines negative for cellular mitochondrial DNA, fused with different platelet partners (platelets contain only mitochondrial DNA and no nuclear DNA). Each cybrid cell line has a combination of SNPs in mitochondrial DNA, relevant to a variety of diseases. Cybrid cell line panel has potential use for drug testing
- Additional notes: Parental cell line was purchased from ECACC
Applications
- Application notes: SNPs characteristic of assigned haplogroup : 257G 263G 477C 750G 1438G 3010A 4769G 8473C 8860G 15326G Additional SNPs 195C 12966T 16519C
Handling
- Growth medium: DMEM high-glucose medium (glucose; 25?mM) supplemented with FBS (10 % v/v), L-glutamine (4?mM), sodium pyruvate (1?mM), and HEPES (1?mM)
- Temperature: 37° C
- Atmosphere: 5% CO2
- Subculture routine: Cells are subcultured weekly (1:12) via trypsinisation.
