UB-UE1 Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: UB-UE1 Cell Line
• Alternate name: UB-UE1; UB/UE-1; University of Bristol/Utricular Epithelium-1; Utricular Epithelium cell line number 1
• Research fields: Developmental biology;Drug development;Genetics
• Parental cell: Pure utricular sensory epithelia of 2-day old immortomouse pups
• Organism: Mouse
• Disease: Hearing loss
• Model: Immortalised Line
• Conditional: Yes
• Conditional description: Cells derived from C57 Bl6 mice carrying a stable insertion of the conditional immortalising gene H-2Kb-tsA58, which describes a temperature-sensitive variant of the SV40 immortalising gene that encodes the large tumour antigen under the control of the gamma interferon-sensitive MHC Class 1 promoter. The transgenic mouse is called the ImmortomouseTM (Jat et al 1991 Proc. Nat. Acad. Sci. USA 88, 5096-5100)
• Description: Derived from vestibular epithelium (utricular macula) using thermolysin treatment and dissection to ensure origin from sensory epithelial supporting cells at post-natal day P2. At this stage the supporting cells are normally post-mitotic but retain the ability to differentiate as sensory hair cells and or supporting cells. UB/UE-1 was characterised by timed expression under differentiating conditions in vitro of a combination of gene and protein markers for epithelial cells and for inner ear sensory cells. These include Cytokeratin, Vimentin, Myosin VIIa, Myosin VI, Brn3c, alpha9AChR and a combination of functional ion channels (see references). Screened with Affymetrix mouse Micro-arrays. The cells differentiate neonatal stage hair cell and supporting cell phenotypes under differentiating conditions in vitro
• Application: Inner ear development; Gene expression and function of inner ear-specific genes; In vitro screening for gene activation and promoter analysis; Ototoxicity studies (prescribed drugs and agents that ameliorate their affects)
• Biosafety level: 1
• Cellosaurus id: CVCL_D807

Applications

• Application: Inner ear development; Gene expression and function of inner ear-specific genes; In vitro screening for gene activation and promoter analysis; Ototoxicity studies (prescribed drugs and agents that ameliorate their affects)

Handling

• Format: Frozen
• Volume: 1 ml
• Growth medium: MEM with 10% FCS, 50Units/ml y-IFN, L-glutamine,
• Temperature: 33° C
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Mycoplasma free: Yes

References

• Hackett et al. 2002. Exp Cell Res. 278(1):19-30. PMID: 12126954
• Lawlor et al. 1999. J Neurosci. 19(21):9445-58. PMID: 10531448