TOV-3291G cell line

Cat. #161784

TOV-3291G cell line

Cat. #: 161784

Organism: Human

Tissue: Derived from high-grade serous tumors


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Inventor: Anne-Marie Mes-Masson and Diane Provencher

Institute: Centre Hospitalier de L’université de Montréal

Primary Citation: Fleury et al. 2015. Genes & Cancer. 6(9-10):378-398. PMID: 26622941

Tool Details

Tool Details


  • Name: TOV-3291G cell line
  • Alternate name: TOV-3291G
  • Organism: Human
  • Gender: Female
  • Tissue: Derived from high-grade serous tumors
  • Donor: Age of diagnosis: 59; BRCA hereditary status: negative Grade 3 – Stage IIIC; Mutations: TP53, CDK12; Pre-treatment
  • Morphology: Formed loosely compact spheroids with irregular margins
  • Growth properties: Adherent
  • Crispr: No
  • Receptors of note: No
  • Description: Epithelial ovarian cancer cell lines spontaneously derived from high-grade serous solid tumors. TOV3291G harbors a TP53 mutation, a comlex CNA pattern, and has a CDK12 mutation.
  • Production details: Established using the Scrape method where tumor tissue was scraped into a 100mm plate with complete OSE medium and maintained for 40 days with medium replaced weekly.
  • Additional notes: Patient 3291 was age 59 at diagnosis and BRCA hereditary status was negative. Their first line treatment was surgery followed by cisplatin/taxol. No previous personal history of cancer and yera of sampling was 2007.


  • Growth medium: OSE medium contains 10% FBS, 0.5ug/mL amphotericin B and 50 ug/mL gentamicin
  • Atmosphere: Low oxygen conditions of 7% O2 and 5% CO2
  • Cultured in antibiotics: Amphotericin B and Gentamicin


  • Vias et al. 2023. Elife. 11
  • 12:e83867. PMID: 37166279 Sevinyan et al. 2022. Cancers (Basel). 16
  • 14(22):5628. PMID: 36428724 Asare-Werehene et al. 2023. Cancers (Basel). 30
  • 15(9):2566. PMID: 37174032