#152109

T47D/S2 Cell Line

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Cat. #152109

T47D/S2 Cell Line

Cat. #: 152109

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Breast

Disease: Cancer

Model: Tumour line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Anne Lykkesfeldt

Institute: Danish Cancer Society

Primary Citation: Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: T47D/S2 Cell Line
  • Cancer: Breast cancer
  • Cancers detailed: Breast carcinoma
  • Research fields: Cancer;Drug development
  • Tool sub type: Continuous
  • Parental cell: T47D
  • Organism: Human
  • Gender: Female
  • Tissue: Breast
  • Disease: Cancer
  • Model: Tumour line
  • Conditional: Yes
  • Description: The T47D/S2 cell line is a control cell line for the tamoxifen resistant T47D/TR-1 and T47D/TR-2 cell lines. This cell line has been established by the reduction of the serum concentration in order to enable growth inhibiton with tamoxifen. T47D/S2 is adherent and the morphology is polygonal epithelial. T47D/S2 cells express oestrogen receptor (alpha) and progesterone receptor. This cell line allows the study of the mechanisms involved in tamoxifen resistant breast cancer cell growth.
  • Application: Determining molecular mechanisms around tamoxifen resistance
  • Production details: T47D/S2 cells have been established by reduction of the serum concentration to 2% fetal calf serum, in order to enable growth inhibition with tamoxifen.
  • Biosafety level: 1
  • Cellosaurus id: CVCL_1D45

Target Details

  • Target: Oestrogen receptor

Applications

  • Application: Determining molecular mechanisms around tamoxifen resistance
  • Application notes: T47DS2 cells express ER alpha and progesterone receptor.

Handling

  • Format: Frozen
  • Passage number: Passage 151 (AL3707, AL3708)
  • Growth medium: Phenol red free RPMI 1640 + 2% FCS + glutamax + 8ug Insulin/ml
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

Related Tools

  • Related tools: T47D/TR-1 Cell Line ; T47D/TR-2 Cell Line ; T47D-182R2 Cell Line ; T47D-182R1 Cell Line

References

  • Larsen et al. 2015. PLoS One. 10(2):e0118346. PMID: 25706943.
  • Larsen et al. 2015. BMC Cancer. 15(1):1-15. PMID: 25885472.
  • Thrane et al. 2014. Oncogene. 34(32):4199-4210. PMID: 25362855.
  • Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.