T47D/TR-2 Cell Line

The T47D/TR-2 Cell line is a breast cancer cell line resistant to tamoxifen.

Contributors

Inventor Institute
Anne Lykkesfeldt Danish Cancer Society, Denmark
Cat. #: 152110
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Breast
Gender: Female
Model: Cancer cell line
Growth properties: Adherent
Cancer Types In Detail: Breast carcinoma;Tamoxifen resistant
Primary citation: Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.
Product description: The T47D/TR-2 cell line is a tamoxifen-resistant derivative of the T47D human breast cancer cell line. It was established, alongside its sister line T47D/TR-1, by adapting T47D/S2 cells to 1 μM tamoxifen under reduced serum conditions (2% FCS), resulting in stable resistance to tamoxifen.
Gender: Female
Conditional: Yes
Production details: Human breast cancer cell line derived from T47D/S2 cells by long-term culture in the presence of 1 μM tamoxifen under reduced serum conditions to induce tamoxifen resistance. Clonal selection was performed in medium without tamoxifen. After 10 months in the presence of tamoxifen, the growth rate of the resistant cell lines had increased to a similar level as the parental cell line.
Cellosaurus ID: CVCL_1D37
Parental cell line: T47D/S2
Disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Phenol red free RPMI 1640 + 2% FCS + glutamax + 8ug Insulin/ml + 1 uM tamoxifen. Fetal Calf Serum (FCS) typically contains less estrogen than Fetal Bovine Serum (FBS) and is the preferred supplement for this cell line.
Subculture routine: Following thawing and counting, dilute the cell suspension with sufficient medium and distribute 5 mL each into T25 flasks to achieve a seeding density of 1.8 – 2.0 x 10^4 / cm2. Place in 37°C, 5% CO₂ incubator. Change medium after 24 hours to remove residual DMSO and then every 2-3 days. Subculture routine: Split 1:7 weekly (slow growing cell line) with Trypsin-EDTA for detachment at 37 °C for 5 minutes. Please also see detailed protocol within the Product Datasheet in the Documentation section below.
Temperature: 37° C
Atmosphere: 5% CO2
Storage medium: 10% DMSO in FCS
Mycoplasma free: Yes
Biosafety level: 1
References:

Larsen et al. 2015. PLoS One. 10(2):e0118346. PMID: 25706943.

Larsen et al. 2015. BMC Cancer. 15(1):1-15. PMID: 25885472.

Thrane et al. 2014. Oncogene. 34(32): 4199-4210. PMID: 25362855.

Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.

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Adapted Larsen et al. 2015. PLoS One. 10(2)

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