Cat. #152109
T47D/S2 Cell Line
Cat. #: 152109
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 8-10 weeks
Organism: Human
Tissue: Breast
Disease: Cancer
Model: Tumour line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Anne Lykkesfeldt
Institute: Danish Cancer Society
Primary Citation: Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: T47D/S2 Cell Line
- Cancer: Breast cancer
- Cancers detailed: Breast carcinoma
- Research fields: Cancer;Drug development
- Tool sub type: Continuous
- Parental cell: T47D
- Organism: Human
- Gender: Female
- Tissue: Breast
- Disease: Cancer
- Model: Tumour line
- Conditional: Yes
- Description: The T47D/S2 cell line is a control cell line for the tamoxifen resistant T47D/TR-1 and T47D/TR-2 cell lines. This cell line has been established by the reduction of the serum concentration in order to enable growth inhibiton with tamoxifen. T47D/S2 is adherent and the morphology is polygonal epithelial. T47D/S2 cells express oestrogen receptor (alpha) and progesterone receptor. This cell line allows the study of the mechanisms involved in tamoxifen resistant breast cancer cell growth.
- Application: Determining molecular mechanisms around tamoxifen resistance
- Production details: T47D/S2 cells have been established by reduction of the serum concentration to 2% fetal calf serum, in order to enable growth inhibition with tamoxifen.
- Biosafety level: 1
- Cellosaurus id: CVCL_1D45
Target Details
- Target: Oestrogen receptor
Applications
- Application: Determining molecular mechanisms around tamoxifen resistance
- Application notes: T47DS2 cells express ER alpha and progesterone receptor.
Handling
- Format: Frozen
- Passage number: Passage 151 (AL3707, AL3708)
- Growth medium: Phenol red free RPMI 1640 + 2% FCS + glutamax + 8ug Insulin/ml
- Temperature: 37° C
- Atmosphere: 5% CO2
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
Related Tools
- Related tools: T47D/TR-1 Cell Line ; T47D/TR-2 Cell Line ; T47D-182R2 Cell Line ; T47D-182R1 Cell Line
References
- Larsen et al. 2015. PLoS One. 10(2):e0118346. PMID: 25706943.
- Larsen et al. 2015. BMC Cancer. 15(1):1-15. PMID: 25885472.
- Thrane et al. 2014. Oncogene. 34(32):4199-4210. PMID: 25362855.
- Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.
