Cat. #161919
SK-MEL-31 Cell Line
Cat. #: 161919
Organism: Human
Tissue: Skin
Model: Tumourigenic
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Lloyd J. Old, Toshitada Takahashi
Institute: Memorial Sloan-Kettering Cancer Center (MSK)
Primary Citation: Carey et al. 1976. Proceedings of the National Academy of Sciences. 73: 3278-3282. PMID: 1067619.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: SK-MEL-31 Cell Line
- Cancer: Skin cancer
- Organism: Human
- Gender: Female
- Tissue: Skin
- Donor: This cell line was established from the tumor cells of a female, of unknown age and ethnicity, with malignant melanoma.
- Morphology: Epithelial
- Growth properties: Adherent
- Model: Tumourigenic
- Model description: Tumourigenic in nude mice; produces malignant melanoma
- Products or characteristics of interest: Karyotype: modal number = 82; range = 74 to 84. This is a hypotetra-/hypertriploid human cell line. The modal chromosome number is 82 occurring at 16% with polyploidy at 10.3%. The markers del(1)(q23), t(1q10p), del(7)(p13), t(7p18q), i(21q) and 5 others are common to all cells. They are all paired in most cells. N1 and N18 are absent, N11 is single-copied, and N6, N7, N8 and N16 are generally 4-copied (many cells also had 5 copies each for N7 and N8). The X is paired. Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; PGM1, 1; PGM3, 1
- Description: SK-MEL-31 is one of a series of melanoma cell lines established from patient-derived tumour samples. This cell line expresses wildtype B-Raf and wildtype N-Ras.
- Application: 3D cell culture
- Biosafety level: 1
Target Details
- Target: Antigen expression: HLA A3,A29, Bw35,B40, Cw3,Cw4, DRw4
Applications
- Application: 3D cell culture
Handling
- Growth medium: EMEM supplemented with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 85%; fetal bovine serum, 15%
- Temperature: 37° C
- Atmosphere: 5% CO2 in air
- Shipping conditions: Dry Ice
- Storage medium: Complete growth medium supplemented with 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Str profiling: Amelogenin: XCSF1PO: 10D13S317: 12D16S539: 13D5S818: 11,13D7S820: 10TH01: 6,9TPOX: 8,11vWA: 16,18D3S1358: 17,18D21S11: 30,32.2D18S51: 15Penta_E: 12,18Penta_D: 9D8S1179: 12,14FGA: 24D19S433: 13,14.2D2S1338: 18,20
References
- Xing et al. 2012. Oncogene. 31(4):446-457. PMID: 21725359.
