SK-MEL-3 Cell Line

SK-MEL-3 is one of a series of melanoma cell lines established from patient-derived tumour samples. This cell line is known to form tumours in immunocompromised mice.

Contributors

Inventor Institute
Lloyd J. Old, Germain Trempe Memorial Sloan-Kettering Cancer Center (MSK)
Cat. #: 161918
Cancer types: Skin cancer
Organism: Human
Tissue: Skin
Gender: Female
Model: Tumourigenic
Morphology: Fibroblast
Growth properties: Adherent
Primary citation: Fogh et al. 1977. Journal of the National Cancer Institute. 59: 221-226. PMID: 327080.
Product description: SK-MEL-3 is one of a series of melanoma cell lines established from patient-derived tumour samples. This cell line is known to form tumours in immunocompromised mice.
Gender: Female
Model description: Tumourigenic in nude mice; forms pigmented malignant melanoma
Products or characteristics of interest: Karyotype: (P13) hypotetraploid to hypertetraploid with abnormalities including dicentrics, pulverizations, secondary constrictions and minutes. Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; PGM1, 1-2; PGM3, 1
Storage conditions: Vapor phase of liquid nitrogen
Shipping conditions: Dry Ice
Growth medium: McCoy's 5a Medium Modified supplemented with FBS to a final concentration of 15%
Subculture routine: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the
Temperature: 37° C
Atmosphere: 5% CO2 in air
Storage medium: Complete growth medium supplemented with 5% (v/v) DMSO
Biosafety level: 1
References:

Fogh et al. 1977. Journal of the National Cancer Institute. 59: 221-226. PMID: 327080.

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