Cat. #161933
SK-CO-1 Cell Line
Cat. #: 161933
Availability: 8-10 weeks
Tissue: Large intestine; Colon
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Lloyd J. Old, Germain Trempe
Institute: Memorial Sloan-Kettering Cancer Center (MSK)
Primary Citation: Fogh et al. 1977. Journal of the National Cancer Institute. PMID: 833871.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: SK-CO-1 Cell Line
- Cancer: Digestive / Gastrointestinal cancer
- Tissue: Large intestine; Colon
- Donor: This cell line was established in 1972 from a metastatic site (ascites) in a 65-year-old Caucasian male with colorectal adenocarcinoma.
- Morphology: Epithelial
- Growth properties: Adherent
- Products or characteristics of interest: Karyotype: (P7) hypertriploid to hypotetraploid with abnormalities including dicentrics, minutes, rings, secondary constrictions, and 8 large submetacentric markers. Isoenzymes: AK-1, 1-2 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1-2
- Description: SK-CO-1 is a human colorectal adenocarcinoma cell line that displays epithelial morphology and grows in adherent tissue culture.
- Application: 3D cell culture
- Biosafety level: 1
Target Details
- Target: Antigen expression: Blood Type O; Rh+; HLA A1, A3, B7, B13
Applications
- Application: 3D cell culture
Handling
- Growth medium: McCoy's 5a Medium Modified with fetal bovine serum to a final concentration of 10%.
- Temperature: 37° C
- Atmosphere: 5% CO2 in air
- Shipping conditions: Dry Ice
- Storage medium: Complete growth medium supplemented with 5% DMSO
- Str profiling: Amelogenin: X,YCSF1PO: 13D13S317: 14D16S539: 13D5S818: 9,11D7S820: 8,10TH01: 8,9TPOX: 8,11vWA: 18,19D3S1358: 17,18D21S11: 28,30D18S51: 19Penta_E: 13,16Penta_D: 11D8S1179: 12,14FGA: 20,25D19S433: 12,13D2S1338: 17,21
References
- Trainer et al. 1988. International journal of cancer. 41(2):287-296. PMID: 3338874.
