Cat. #161931
Saos-2 Cell Line
Cat. #: 161931
Availability: 8-10 weeks
Tissue: Bone tissue
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Jorgen Fogh
Institute: Memorial Sloan-Kettering Cancer Center (MSK)
Primary Citation: Fogh et al. 1977. Journal of the National Cancer Institute. 58.2.209. PMID: 833871.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Saos-2 Cell Line
- Cancer: Sarcoma
- Gender: Female
- Tissue: Bone tissue
- Donor: This cell line was established in 1973 from an 11-year-old Caucasian female with osteogenic sarcoma.
- Morphology: Epithelial
- Growth properties: Adherent
- Products or characteristics of interest: Karyotype: The stemline chromosome number is hypotriploid with the modal number of 56 chromosomes per cell and the 2S component occurring at 13.2%. Over two-thirds of the chromosome complement consisted of structurally rearranged chromosomes., Most marker chromosomes had complex rearrangements. The origin of the segments composing these markers could not be identified. Of the identifiable markers, 6p+/q+, 7p+, 11p+, and 12p+ occasionally were present at 2 copies per cell., The Y chromosome was not detected in the QM stained preparation. Isoenzymes: AK-1, 1; ES-D, 2; G6PD, B; GLO-I, 2; Me-2, 1; PGM1, 1-2; PGM3, 1-2
- Description: Saos-2 is a human osteosarcoma cell line, which displays several osteoblastic features.
- Application: 3D cell culture; Cancer research
- Biosafety level: 1
Target Details
- Target: Antigen expression: Blood Type B, Rh+; HLA A2, A3, Bw16, Bw47
Applications
- Application: 3D cell culture; Cancer research
Handling
- Growth medium: McCoy's 5a Medium Modified with fetal bovine serum to a final concentration of 10%.
- Temperature: 37° C
- Atmosphere: 5% CO2 in air
- Shipping conditions: Dry Ice
- Storage medium: Complete growth medium supplemented with 5% DMSO
- Str profiling: Amelogenin: D3S1358: 14,18TH01: 6,9D21S11: 28,30D18S51: 15,22Penta_E: 14,19D5S818: 12D13S317: 12,13D7S820: 8,10D16S539: 12,13CSF1PO: 10Penta_D: 11,12Amelogenin: XvWA: 18D8S1179: 10,12TPOX: 8FGA: 22,25D19S433: 13D2S1338: 18
References
- MM et al. 1985. Virology. 145.2. PMID: 2992153.
- Yee et al. 1985. The American journal of pathology. 361-366. PMID: 2990217.
