RPE1 PBRM1 KO2

RPE1-hTERT cell line knockout for PBRM1 (clone 2, also called B16)

Contributors

Inventor Institute
Jessica Downs, Karen Lane The Institute of Cancer Research
Cat. #: 161857
Organism: Human
Tissue: retinal epithelium
Gender: Female
Model: Genetically modified ; immortalised non-cancer cells
Morphology: Epithelial
Growth properties: Adherent
Product description: RPE1-hTERT cell line knockout for PBRM1 (clone 2, also called B16). This cell line was generated using CRISPR-Cas9 engineering to create a loss of function mutation in the indicated subunit of the SWI/SNF chromatin remodelling complex. The cell line was validated using Sanger sequencing, immunofluorescence, and proteomic analysis.
Gender: Female
CRISPR: Yes
Conditional: No
Cellosaurus ID: CVCL_4388
Parental cell line: RPE1-hTERT
Model description: PBRM1 knockout
Products or characteristics of interest: Gene loss-of-function, chromatin-remodelling, DNA damage, cell cycle response, regulation of t-cell-mediated cell killing.
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM /Ham F12 nutrient mixture + 10S + 1% Penicillin/Streptomycin
Temperature: 37C
Atmosphere: 5% CO2 in air
Biosafety level: 1
References:

Unpublished

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