MHYO-LCL1 Pre-leukaemic (DNMT3a R882H mutant) Human B-lymphoblast Cell Line

THe MYHO-LCL cell line is very unique since it carries the pre-leukaemia DNMT3a R882H mutation, and therefore is useful in the investigation of B lymphocyte lineages.

Contributors

Inventor Institute
MIchal Hayun Rambam Health Care Campus
Cat. #: 160591
Tool sub type: Primary
Unit size: 1×10^6 cells / vial
Cancer types: Blood cancer
Research Fields: Cancer
Organism: Human
Tissue: Blood
Model: Tumour line
Growth properties: Confirmed antigen expression: CD19, CD20, CD27, CD45, HLA-DR, CD49d. Cytokine secretion: IL-10
Cancer Types In Detail: Leukaemia;Pre-leukaemia
Product description: THe MYHO-LCL cell line is very unique since it carries the pre-leukaemia DNMT3a R882H mutation, and therefore is useful in the investigation of B lymphocyte lineages. The cell line phenotype is of memory B lymphocytes, and therefore is relevant to B-cell development, oncology, and immunology. Relevant research areas include: Drug discovery, B-cell biology, B-cell development and transformation, aging, and acute myeloid leukaemia.These cells are monoclonal as confirmed by IGH gene clonality assay.
Conditional: No
Production details: This cell line was spontaneously grown from peripheral blood drawn from a male acute myeloid leukaemia patient upon diagnosis. This line was established in culture four weeks after seeding the AML blast cells. The cells contain low EBV mRNA expression and secret EBV into media, confirming immortalisation and transformation. These cells are in suspension and mostly grow as aggregates.
Parental cell line: Novel patient-derived (male; acute myeloid leukaemia) cell line
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Cells have been grown in IScove Modified Dulbecco's Medium containing 10% foetal bovine serum and 1% pencicillin/streptomycin. Cells are grown at 37 degrees celsius and 5% CO2. Cultures were split at confluency 1:2 or 1:3 two or three times per week.

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