#151774

MEF ULK1 ULK2 DKO (SIM) Cell Line

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Cat. #151774

MEF ULK1 ULK2 DKO (SIM) Cell Line

Cat. #: 151774

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Mouse

Tissue: Embryo

Disease: Cancer

Model: Knock-Out

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Sharon Tooze

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: MEF ULK1 ULK2 DKO (SIM) Cell Line
  • Research fields: Apoptosis and autophagy;Cancer;Cell signaling and signal transduction;Metabolism;Neurobiology
  • Organism: Mouse
  • Tissue: Embryo
  • Disease: Cancer
  • Growth properties: Fibroblast
  • Model: Knock-Out
  • Conditional: Yes
  • Description: The MEF ULK1 ULK2 DKO (SIM) cell line provides a tool for the study of Ulk1 and Ulk2 and of Autophagy. Mouse embryonic fibroblast Ulk1/Ulk2 double knock-out cell line is from an embryo with a mixed genetic background (Blk6/129 Agouti).
  • Production details: Primary embryonic fibroblasts were isolated from the embryos of a pregnant female Ulk1-/- Ulk2-/+ mouse at day 13 p.c. The embryos were genotyped to identify those that were Ulk1 -/- Ulk2 -/- and the MEFs that were isolated and cultured were immortalised by SIM using a standard serial passaging protocol.
  • Biosafety level: 1
  • Cellosaurus id: CVCL_5A56

Target Details

  • Target: ULK1, ULK2

Handling

  • Format: Frozen
  • Growth medium: DMEM + 20% FCS + 2mM Glutamine + pen/strep
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

Related Tools

  • Related tools: MEF ULK1 KO (SIM) Cell Line ; MEF ULK1/2 WT (SIM) Cell Line ; MEF ULK2 KO (SIM) Cell Line ; MEF ULK1 ULK2 DKO (SV40) Cell Line ; MEF ULK1/2 WT(SV40) Cell Line ; MEF ULK1 KO (SV40) Cell Line ; MEF ULK2 KO (SV40) Cell Line

References

  • McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.
  • Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.