MEF ULK1 ULK2 DKO (SIM) Cell Line

Discover DKO, a powerful cell line with knockout of two genes, and learn more about its applications and properties on CancerTools.org product page.

Contributors

Inventor Institute
Sharon Tooze Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151774
Unit size: 1×10^6 cells / vial
Research Fields: Apoptosis and autophagy;Cancer;Cell signaling and signal transduction;Metabolism;Neurobiology
Organism: Mouse
Tissue: Embryo
Model: Knock-Out
Growth properties: Fibroblast
Alternate name: MEF Ulk1 -/- Ulk2 -/- (DKO) (SIM)
Product description: MEF ULK1 ULK2 DKO (SIM) is a spontaneously immortalised cell line. This mouse embryonic fibroblast Ulk1/Ulk2 double knock-out cell line is from an embryo with a mixed genetic background (Blk6/129 Agouti).The MEF ULK1 ULK2 DKO (SIM) cell line provides a tool for the study of Ulk1 and Ulk2 and of autophagy. Autophagy has been implicated in cancer, neurodegeneration and immune-related disorders. Discover our group of mouse embryonic fibroblast cell lines for autophagy research. 
Conditional: Yes
Production details: Primary embryonic fibroblasts were isolated from the embryos of a pregnant female Ulk1-/- Ulk2-/+ mouse at day 13 p.c. The embryos were genotyped to identify those that were Ulk1 -/- Ulk2 -/- and the MEFs that were isolated and cultured were immortalised by SIM using a standard serial passaging protocol.
Cellosaurus ID: CVCL_5A56
disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM + 20% FCS + 2mM Glutamine + pen/strep
Mycoplasma free: Yes
Biosafety level: 1
References:

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.

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