MEF Raf1 FF/FF KI Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.

MEF Raf1 FF/FF KI Cell Line. Late log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.

Cat. #151629

MEF Raf1 FF/FF KI Cell Line

Cat. #: 151629

Unit size: 1x10^6 cells / vial

Availability: 8-10 weeks

Organism: Mouse

Tissue: Embryo

Disease: Cancer

Model: Knock-In

£575.00

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Contributor

Inventor: Catrin Pritchard

Institute: University of Leicester

Tool Details

Target Details

Applications

Handling

Related Tools

References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

Name: MEF Raf1 FF/FF KI Cell Line
Alternate name: Raf-1FF/FF MEF; MEF Raf1 FF/FF KI
Research fields: Cancer;Cell signaling and signal transduction;Drug development;Metabolism
Parental cell: See the Raf KI FF knockout mouse that these cells were derived from in related products.
Organism: Mouse
Tissue: Embryo
Disease: Cancer
Model: Knock-In
Conditional: Yes
Description: The MEF Raf1 FF/FF KI Cell Line is a MEF line obtained from the knock-in mouse carrying inactive Raf-1FF/FF mutant.
Production details: Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4ÄÂ?Â°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture.
Biosafety level: 1
Cellosaurus id: CVCL_6D90

Target Details

Target: Raf1 Y340F Y341F inactive mutant

Applications

Application notes: See the Raf KI FF knockout mouse that these cells were derived from in related products.

Handling

Format: Frozen
Growth medium: DMEM, 10% FCS, 100U/mol penicillin/streptomycin
Unit size: 1x10^6 cells / vial
Shipping conditions: Dry ice
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Mycoplasma free: Yes

Related Tools

Related tools: Raf-1FF/FF knockin mouse

References

Hser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
MEK kinase activity is not necessary for Raf-1 function.

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