Cat. #156437
MDA-MB-231 CAGA12-Luc/TK Renilla cell line
Cat. #: 156437
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 8-10 weeks
Organism: Human
Tissue: Breast
Disease: Cancer
Model: Reporter
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Caroline Hill
Institute: The Francis Crick Institute
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: MDA-MB-231 CAGA12-Luc/TK Renilla cell line
- Cancer: Breast cancer
- Cancers detailed: Breast cancer
- Research fields: Cancer;Cell signaling and signal transduction;Developmental biology
- Tool sub type: Continuous
- Parental cell: MDA-MB-231 cell line
- Organism: Human
- Tissue: Breast
- Disease: Cancer
- Model: Reporter
- Conditional description: In the presence of activin and TGF-beta signalling luciferase is expressed.
- Description: The human breast cancer cell line MDA-MB-31 modified for use as a reporter cell line for Activin and TGF-beta signalling. The cell line stably expresses CAGA12-Luc, a Smad3/Smad4-dependent reporter containing 12 copies of the CAGAC sites (AGAC motif underlined) from the PAI-1 promoter. This is a Smad3/Smad4-dependent reporter, although it also binds complexes of Smad2 exon 3 with Smad4. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control.
- Production details: Made by transfecting the breast cancer cell line MDA-MB-231 with CAGA12-Luc with pRetroSuper for puromycin resistance and then TK Renilla with pCMV-Bsd for blasticidin resistance.Clonal
- Biosafety level: 1
- Recommended controls: MDA-MB-231 parental line
Target Details
- Target: Activin and transforming growth factor ÄÂ? (TGF-ÄÂ?Â)ÄÂ? signalling
Handling
- Format: Frozen
- Growth medium: Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)+ 50 ?g/ml blasticidin
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
Related Tools
- Related tools: HEK293T CAGA12-Luc/TK Renilla cell line
References
- Grnroos et al. 2012. Mol Cell Biol. 32(14):2904-16. PMID: 22615489.
